穿膜肽R6促进低分子肝素大鼠肠道吸收的研究

Intestinal Absorption Studies on Low Molecular Weight Heparin in Rats Using Cell-penetrating Peptide R6 as Absorption Enhancer

关键词:  低分子肝素  穿膜肽  六聚精氨酸

Key words:  low molecular weight heparin  cell-penetrating peptide  R6

摘要:

目的 研究穿膜肽R6促进低分子肝素大鼠肠道吸收的作用。方法 以用药前后血液凝固时间的变化为指标，采用大鼠十二指肠给药的方法评价R6对低分子肝素的促吸收作用，采用肠袢法研究R6的主要促吸收部位。结果 R6作为吸收促进剂，低分子肝素十二指肠给药后凝血时间显著延长，R6在十二指肠、空肠、回肠部位均显示促吸收作用，且在回肠部位的促吸收效果最显著。结论 R6能够显著促进低分子肝素的大鼠肠道吸收。

Abstract:

OBJECTIVE To study the intestinal absorption of low molecular weight heparin (LMWH) in rats using cell-permeable peptides R6 as absorption enhancer. METHODS The intestine absorption enhancing effect of R6 on LMWH was determined by the changes of blood coagulation time after intraduodenal administration to rats. Intestinal loops were used to identify and compare the main segments of intestines responsible for the absorption of LMWH using R6 as absorption enhancer. RESULTS Blood coagulation time was significantly prolonged after intraduodenal administration of LMWH to rats using R6 as absorption enhancer. R6 enhanced LMWH absorption in the regions of the duodenum, jejunum and ileum of rats and the absorption enhancing effect was the most noticeable in the ileum. CONCLUSION R6 had a significant intestinal absorption enhancing effect on LMWH.