河南产山橿醋酸乙酯部位HPLC指纹图谱研究

Study on HPLC Fingerprint of Ethyl-acetate Part of Radix Lindera Reflexae in Henan Province

关键词:  山橿  醋酸乙酯部位  高效液相色谱法  指纹图谱

Key words:  Radix lindera reflexae  ethyl acetate part  HPLC  fingerprint

摘要:

目的建立山橿醋酸乙酯部位的HPLC色谱指纹图谱。方法采用高效液相色谱法，色谱条件：依利特Hypersil ODS2 C18(250 mm×4.6 mm，5 μm)色谱分析柱；流动相：甲醇-水梯度洗脱；检测波长297 nm；柱温30 ℃；流速1.0 mL·min-1。结果本试验在测定了23批山橿药材醋酸乙酯部位的HPLC色谱指纹图谱的基础上，建立了山橿药材醋酸乙酯部位的HPLC色谱指纹图谱，并进行了相似度分析，除了河南省西峡县产山橿药材的相似度比较低外，其余相似度都在0.85以上。结论本方法简便、快捷、可靠，可用于山橿药材的质量控制。

Abstract:

OBJECTIVE To establish HPLC fingerprint of ethyl acetate part of Radix linderae reflexae. METHODS HPLC method was used. Chromatographic column was a Hypersil ODS2 C18(250 mm ×4.6 mm，5 μm); mobile phase was water-methanol with gradient elution；detected wavelength was 297 nm; column temperature was 30 ℃; flow rate was 1.0 mL·min-1. RESULTS The HPLC fingerprint of ethyl acetate part of Radix linderae reflexae was established based on the determination of 23 batches of Radix linderae reflexae, and the similarity was analysized. The similarity evaluation is above 0.85 except the sample S13 collected from Xixia country in Henan province. CONCLUSION The method is simple and reliable, and can be used for control the quality of Radix linderae reflexae.