芦荟苷预处理对缺血再灌注大鼠心肌细胞凋亡、TNF-α含量和Ca2+-ATPase活性的影响

Effects of Barbaloin Pretreatment on Myocardial Cell Apoptosis, the Contents of TNF-α and Activity of Ca2+-ATPase in Rats with Ischemia Reperfusion

关键词:  芦荟苷  再灌注损伤  细胞凋亡  TNF-α  Ca2+-ATPase

Key words:  Barbaloin  reperfusion injury  apoptosis  TNF-α  Ca2+-ATPase

摘要:

目的研究芦荟苷(Barbaloin，Barb)对急性心肌缺血再灌注损伤大鼠心肌细胞凋亡的影响。方法结扎大鼠左冠状动脉前降支复制心肌缺血再灌注损伤模型，实验分为假手术组、缺血再灌注组(IR)、Barb高、低剂量预处理组。采用原位缺口末端标记法(TUNEL)检测心肌细胞凋亡指数(AI)，ELISA法测定血清TNF-α水平，化学法测定线粒体Ca2+-ATPase活性。结果与IR组比较，Barb组AI和TNF-α水平显著降低 (P<0.05或P<0.01)，Ca2+-ATPase活性明显增高(P<0.05)。结论Barb能明显抑制IR引起的心肌细胞凋亡，其作用可能与降低TNF-α水平和提高Ca2+-ATPase活性有关。

Abstract:

Objective To observe the effects of Barbaloin pretreatment on myocyte apoptosis in rat hearts with acute myocardial ischemia and reperfusion(IR). Methods The left anterior descending branch of coronary artery was ligated to make a myocardial ischemia-reperfusion model in rats. The experiment rats were divided into four groups: sham operation (SO), ischemia-reperfusion (IR), Barbaloin (45,90 mg.kg-1·d-1) group. Apoptosis index (AI) of myocardium was detected by TUNEL in situ apoptosis assay kit, TNF-α were measured by ELISA and Ca2+-ATPase activity of mitochondrial were measured by chemical method. Results Compared with IR group, AI and TNF-α was significantly decreased in Barbaloin group. Moreover, the activity of Ca2+-ATPase was increased in Barbaloin group. Conclusion Barbaloin can significantly inhibit cardiomyocyte apoptosis in ischemia-reperfusion rat hearts. The possible mechanism is to reduce TNF-α levels and raise Ca2+-ATPase activity in rats.