微波辐射-溶剂回流提取农吉利中农吉利甲素

Microwave Irradiation Coupled to Solvent Reflux Extraction of Monocrotaline in Crotalaria Sessiliflora L

关键词:  农吉利  农吉利甲素  高效液相色谱法

Key words:  Crotalaria sessiliflora L  monocrotaline  HPLC

摘要:

目的利用微波辐射引起农吉利药材细胞破壁，研究在微波辐射-溶剂回流提取中各因素对生物碱农吉利甲素提取的影响，探讨其最佳实验条件及工艺方法，以提高其提取效率。方法采用微波辐射处理含有一定水分的药材，再以甲醇为溶剂回流提取农吉利中农吉利甲素，用高效液相方法测定提取液的含量。结果微波辐射-溶剂回流提取农吉利甲素的实验条件为：药材颗粒度80目，加水量21.0 mL，微波功率40%，辐射时间2 min，提取温度80 ℃，提取时间2 h，料液比1∶20。结论经过微波辐射农吉利药材，再溶剂回流提取，农吉利甲素的提取率明显提高，优于直接溶剂回流提取和超声提取方法，且方法简单，操作简便。

Abstract:

OBJECTIVE Through microwave irradiation caused the cells ruptured, and then monocrotaline in Crotalaria sessiliflora L was extracted by solvent reflux method to improve extraction yield of the alkaloid. METHODS The material of crotalaria Sessiliflora L containing a certain amount of water was irradiated by microwave. Using methanol as solvent, monocrotaline was extracted and determined by high performance liquid chromatography. RESULTS Optimum experimental conditions ware: 80 mesh of particle size, 21.0 mL of water volume added, 40% of microwave power, 2 minutes of irradiation time, 80 ℃ of extraction temperature, 2 h of extraction time and 1:20 of ratio of material to solvent. CONCLUSION The extraction of monocrotaline in Crotalaria Sessiliflora L was performed successfully by using microwave irradiation couple to solvent reflux extraction. The alkaloid yield was higher than conventional solvent reflux extraction and ultrasonic extraction, and the method is simple, easy to operate.