大孔树脂同步分离栀子中栀子苷和栀子黄色素工艺研究

Study of Separatation and Purification for Geniposide and Gardenia Yellow from Gardenia Simultaneously with Macroporous Absorption Resin

关键词:  分离纯化  大孔吸附树脂  栀子黄色素  栀子苷

Key words:  separation and purification  macroporous resin  gardenia yellow  geniposide

摘要:

目的研究大孔树脂同步分离纯化栀子药材中的栀子苷和栀子黄色素。方法通过比较不同大孔吸附树脂静态吸附栀子黄色素的能力，并对最佳大孔吸附树脂纯化工艺进行筛选。结果HPD-100型树脂综合性能最佳，其纯化工艺为：上样液浓度A440在60左右，以流样速度1～3 mL·min-1上样；水洗8～10 BV；25%乙醇冲洗3～5 BV体积；70%乙醇冲洗至洗脱液无色。结论栀子苷得率70%、栀子黄色素(色价100～500、OD小于0.8甚至达到0.3)得率80%(折算至栀子药材含量)，说明此工艺可行。

Abstract:

OBJECTIVE To study the separatation and purification for geniposide and gardenia yellow from gardenia simultaneously with macroporous resin. METHODS Comparing the the static adsorption capacity of gardenia yellow was used to investigate different macroporous resin, and get the optimum purified process with the best macroporous resin. RESULTS The best macroporous resin: HPD-100 macroporous resin; and the purify process: the extracted solution(absorbency at 440nm was about 60) was passed through HPD-100 resin chromatographic column with flow rate of 1-3 mL·min-1, after which，eluted it with distilled water (8-10 times volum), 25% ethanol (double volum) and 70% ethanol to be almost colorless. CONCLUSION The percent of yield of geniposide was 70%, and that of gardenia yellow (color price between 100 to 500, the OD less than 0.8 or even 0.3) was 80% (equivalent to the content of medica gardenia), all proves that the technique condition is reasonable.