结肠靶向定位滴丸克癌素的提取工艺研究

Study on Extraction Technique of Medicine Cruing Cancer on Colon-Specific Target Dropping Pill

关键词:  结肠  靶向  克癌素  提取工艺

Key words:  colon  targeting  medicine cruing cancer  extraction process

摘要:

目的 建立结肠靶向滴丸克癌素的提取工艺方法。方法 采用碱溶酸沉法从槐米中提取芦丁，再进行酸水解得到槲皮素；采用传统乙醇回流法从姜黄中提取总姜黄素，以姜黄中的总姜黄素含量为指标，通过正交表分析确定最佳提取工艺。结果 槲皮素的提取条件为：碱性pH 8~9，酸性pH 3~5条件下提取芦丁；得到精制芦丁加100倍量的2%硫酸酸水解90 min。姜黄的最佳提取工艺为：85%乙醇回流提取3次，每次1 h；得浸膏上硅胶柱收集氯仿洗脱部分得总姜黄素。结论 该工艺可行，可为结肠靶向滴丸克癌素的生产提供参考。

Abstract:

OBJECTIVE To set up a method of extraction technique of medicine cruing cancer on colon-specific target dropping pill. METHODS Using alkali-soluble acid ways to extract rutin from flos sophorae immaturus, then using the acid hydrolysis to obtain quercetin. Using traditional ethanol reflux to extract total curcumin from turmeric, using the content of turmeric as the total target, to determine the optimum extraction process through the analysis of orthogonal table. RESULTS Quercetin extraction conditions are as follows: alkaline is pH 8~9, acidic conditions is pH 3~5, extracting rutin, then add 100 times volume of 2% sulfuric acid to hydrolyze 90 minutes. The optimum extraction process of turmeric extraction are as follows: using 85% ethanol extract reflux 3 times, each time is 1 hour. Extracting the part-eluting of chloroform on silica gel column to collect total curcumin. CONCLUSION The process is feasible and supplying reference for production of medicine cruing cancer on colon-specific target dropping pill.