微波辅助萃取马铃薯叶中茄尼醇的工艺研究

Study on Extraction Technology of Solanesol from Potato Leaves by Microwave-Assisted Extraction

关键词:  微波辅助萃取  茄尼醇  马铃薯叶  高效液相色谱法

Key words:  microwave-assisted extraction  solanesol  potato leaves  HPLC

摘要:

目的 研究微波辅助萃取马铃薯叶中茄尼醇的工艺条件。方法 以95%乙醇为提取溶媒，以反相高效液相色谱法为定量测定方法，考察料液比、辐射时间、微波功率、萃取温度和萃取次数等因素对微波辅助萃取马铃薯叶中茄尼醇提取率和浸膏得率的影响，并与索氏提取法进行了比较。结果 微波辅助萃取马铃薯叶中茄尼醇的最佳工艺条件为：料液比1∶10，微波功率450 W，萃取温度55 ℃，提取2次，每次20 min。结论 应用微波辅助萃取马铃薯叶中的茄尼醇具有萃取时间短，萃取效率高，节约能耗等优点，具有较好的应用前景。

Abstract:

OBJECTIVE To study the extraction technology condition of solanesol from discarded potato leaves by microwave-assisted extraction (MAE) method. METHODS 95% ethanol was used as extraction solvent, the content of solanesol was determined by the reversed phase high performance liquid chromatography (RP-HPLC), the influence of ratio of material to solvent, radiation time, microwave power, temperature and extraction times were studied so as to analyze the factors which effect the extraction yield of solanesol by MAE, the effects of microwave-assisted extraction method was compared with those of soxhlet extraction method. RESULTS The optimum extraction parameters were chosen as follows: the ratio of material to solvent was 1∶10, the microwave power was 450 W, the temperature was 55 ℃, extraction 2 times and 20 minutes for each time. CONCLUSION The results indicate that MAE is fast, efficient, and can save energy consumption, and it has good prospects of application.