树脂理化特性对分离葛根总黄酮的影响

Impact of Physical and Chemical Characteristics of Resin on Separating Total Flavones from Radix Puerariae Lobatae

关键词:  ADS吸附树脂  葛根总黄酮  分离纯化

Key words:  ADS adsorbing resins  total flavones from Radix Puerariae Lobatae  separation and purification

摘要:

目的 研究吸附树脂理化性质对分离葛根总黄酮的影响。方法 采用分光光度法分别对5种ADS系列吸附树脂作静态、动态研究，并对较优树脂进行动态吸附-脱附工艺参数的优化。结果 静态实验表明，树脂的极性和空间结构是影响吸附效果的重要因素；树脂ADS-8上柱液浓度约为0.453 mg·mL^-1，pH 5~6，吸附速率4 BV·h^-1，400 mL蒸馏水洗去杂质，洗脱剂为70%乙醇，洗脱流速2 BV·h^-1，用量为60 mL。结论 ADS-8是分离纯化葛根总黄酮的较优树脂。

Abstract:

OBJECTIVE Study on the impact of the physical and chemical characteristics of resin on separating total flavones from Radix Puerariae Lobatae. METHODS Five kinds of ADS adsorbing resins were tested in the static and dynamic research about separating and purifying total flavones. And the best resin was screened out, then its technical parameters in dynamic adsorption-desorption were optimized. RESULTS The static adsorption tests showed that the polarity and textual structure were main factors on the adsorptive capacity. The optimum conditions for ADS-8 were identified, which were 0.453 mg·mL^-1 corresponding to crude drug for concentration of extract, pH 5-6 with the adsorption speed of 4 BV·h^-1; water volume for removing impurities was 400 mL; and the concentration of the eluant ethanol 70%, volume of ethanol was 60 mL with the desorption speed of 2 BV·h^-1. CONCLUSION ADS-8 is the best resin for separating and purifying total flavones.