赤芍总苷退黄降酶的作用及机制研究

Study on the Jaundice-Relieving and Enzyme-Reducing Effect and Its Mechanism of Total Paeony Glycoside

关键词:  赤芍总苷  退黄降酶  胆汁分泌量  睡眠时间  尿苷二磷酸葡萄糖酸转移酶

Key words:  total paeony glycoside  jaundice-relieving and enzyme-reducing  capacity of bile secretion  sleeping time  UDPG-T

摘要:

目的 探讨赤芍总苷退黄降酶的作用及机制。方法 ICR小鼠随机分成正常对照组，模型对照组，赤芍总苷低、中、高剂量组和苯巴比妥钠组；灌胃α-萘异硫氰酸酯(ANIT)诱导小鼠黄疸模型，分别在实验第6天和第12天观察各组药物对小鼠总胆红素(TBIL)、直接胆红素(DBIL)、胆汁酸(TBA)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)等血清学指标及对小鼠胆汁分泌量和肝组织中尿苷二磷酸葡萄糖酸转移酶(UDPG-T)活性的影响，实验第6天观察各组药物对戊巴比妥钠睡眠时间的影响。结果 与模型对照组比较，除第6天赤芍总苷中剂量组ALT、第12天低剂量组AST无显著差异外，赤芍总苷3个剂量组血清学指标均明显下降(P<0.05或P<0.01)；赤芍总苷中、高剂量组胆汁分泌量显著增加(P<0.01或P<0.05)；3个剂量组和苯巴比妥钠组睡眠时间均明显缩短(P<0.01)；赤芍总苷组肝组织UDPG-T活性明显提高(P<0.05)。结论 赤芍总苷具有退黄降酶作用，其作用机制可能与增加胆汁分泌量、提高肝药酶及UDPG-T活性有关。

Abstract:

OBJECTIVE To research the jaundice-relieving and enzyme-reducing effect and its mechanism of total paeony glycoside (TPG). METHODS All ICR mice were randomly divided into normal group, model group, low dose group of TPG (LG), middle dose group of TPG (MG), high dose group of TPG(HG), and phenobarbital sodium group. Intrahepatic cholestatic model of mice was established after ig alpha-naphthylisothiocyanate (ANIT). Effect of drugs on TBIL, DBIL, TBA, ALT, AST, and ALP in serum, capacity of bile secretion, and uridine diphosphoglucuronyl transferase (UDPG-T) activity of liver tissue were observed on the sixth and twelfth day, respectively. Sleeping time of pentobarbitol sodium were observed on the sixth day. RESULTS Compared with the model group, all serum indices of three TPG groups degraded significantly (P<0.01 or P<0.05), except ALT of MG on the sixth day and AST of LG on the twelfth day. Capacity of bile secretion of MG and HG increased significantly (P<0.01 or P<0.05), sleeping time of pentobarbitol sodium of all three TPG groups shortened obviously (P<0.01), and UDPG-T activity of liver tissue of TPG enhanced significantly (P<0.05). CONCLUSION TPG possesses the jaundice-relieving and enzyme-reducing effect and its mechanism might be related with its effect of increasing capacity of bile secretion, enhancing the activiy of liver drug enzyme and UDPG-T of model mice.