HPLC测定利奈唑胺的血药浓度

HPLC Determination of Linezolid Plasma Concentration

关键词:  利奈唑胺  高效液相色谱法  血药浓度

Key words:  linezolid  HPLC  plasma concentration

摘要:

目的 建立人血浆中利奈唑胺浓度测定的高效液相色谱法。方法 以苯巴比妥为内标，血浆样品用乙腈沉淀蛋白，采用Luna C₁₈柱(250 mm×4.6 mm，5 μm)分析。流动相为乙腈-水(25∶75)，流速为1.0 mL·min^-1，检测波长251 nm，柱温35 ℃。 结果 本法在0.1～40 mg·L^-1内线性关系良好(r=0.999 9)，检测限为0.05 mg·L^-1，日内、日间RSD均小于4％。结论 本方法简单、灵敏、准确，适用于利奈唑胺临床血药浓度监测及人体药动学研究。

Abstract:

OBJECTIVE To establish an HPLC method to determine the concentration of linezolid in human plasma．METHODS With phenobarbital as internal standard, the protein plasma sample was precipitated with acetonitrile．The separation was carried out using Luna C₁₈ (250 mm×4.6 mm, 5 μm) column with mobile phase of acetonitrile-water (25∶75) at a flow rate of 1.0 mL·min^-1．The detection wavelength was 251 nm and the column temperature was 35 ℃．RESULTS The linear range was 0.1-40 mg·L^-1 (r=0.999 9), and the LOQ was 0.05 mg·L^-1. The intra-day and inter-day precision were both less than 4%. CONCLUSION The method is simple, sensitive, accurate and suitable for the study of therapeutic drug monitoring and the pharmacokinetic study of linezolid．