LC-MS检测金锁固精丸中非法添加的枸橼酸西地那非

Determination of Sildenafil Citrate Illegally Mixed into Jinsuo Gujing Pills by LC-MS

关键词:  枸橼酸西地那非  液质联用  检测

Key words:  sildenafil citrate  LC-MS  determination

摘要:

目的 采用液质联用技术检测金锁固精丸中非法添加的枸橼酸西地那非。方法 选用C₁₈色谱柱(2.1 mm×100 mm，5 µm)，以乙腈-0.01 mol·L^-1乙酸铵溶液梯度洗脱，对此制剂的提取液进行液质联用分析，通过与对照品的色谱和质谱行为比较，检测此制剂中枸橼酸西地那非成分。结果 在该金锁固精丸中检出枸橼酸西地那非。结论 本方法简便、灵敏、准确，专属性强，可分析检测此制剂中的枸橼酸西地那非。

Abstract:

OBJECTIVE To detect sildenafil citrate illegally mixed into Jinsuo Gujing pills by LC-MS. METHODS The sildenafil citrate of Jinsuo Gujing pills was analyzed through a C₁₈ column (2.1 mm×100 mm, 5 µm), using the mobile phase of acetonitrile-0.01 mol·L^-1 ammonium acetate by gradient elution. RESULTS Sildenafil citrate were found in the drugs. CONCLUSION The method is simple, sensitive and accurate. It can be used to detect sildenafil citrate mixed into illegal drug.