舒胆通颗粒对实验动物胆汁分泌及胆囊平滑肌的影响

The Effect of Shudantong Granules on Animals' Bile Secretion and Gallbladder Smooth Muscle

关键词:  舒胆通颗粒  胆汁分泌量  胆囊平滑肌

Key words:  Shudantong granules  bile secretion  gallbladder smooth muscle

摘要:

目的 观察舒胆通(SDT)颗粒对大鼠胆汁分泌及胆囊平滑肌的影响。方法 采用在体胆汁引流法，观察单次给予SDT对大鼠胆汁分泌以及总胆红素(TB)的影响；采用离体试验法观察SDT对豚鼠胆囊平滑肌条的直接作用及其对阿托品、酚妥拉明、苯海拉明、雷尼替丁的拮抗作用。结果 SDT 5，10，20 g·kg^-1剂量能使大鼠给药后30~90 min时段胆汁分泌增加，但胆汁中TB水平无明显改变；终浓度为2.5×10^-4，5×10^-4，1×10^-3 mg·mL^-1的SDT能使离体豚鼠胆囊肌条张力增高、收缩频率加快、收缩幅度增加，并呈现明显的量效关系，其兴奋作用可被阿托品完全阻断，也可被酚妥拉明部分阻断，但不能被苯海拉明、雷尼替丁所阻断。结论 SDT能增强胆囊平滑肌的兴奋性，促进胆汁分泌；其利胆作用可能主要经由胆碱能M受体介导，部分经由肾上腺素能α受体介导、而与组胺H1受体、前列腺素受体等途径无关。

Abstract:

OBJECTIVE To observe the effect of Shudantong granules(SDT) on animals' bile secretion and gallbladder smooth muscle. METHODS Biliary drainage method in vivo was applied to detect the bile secretion and total bilirubin (TB) level of rats before and after single administration of SDT; and the direct effect of SDT on gallbladder smooth muscle of guinea pig and its antagonistic effect on atropine, phentolamine, diphenhydramine and ranitidine were observed in vitro. RESULTS SDT can increase bile secretion of rats at the dosage of 5, 10, 20 g·kg^-1 within 30-90 min after administration, but the level of TB in bile had no significant change; SDT can also enhance the strain, contraction range and contraction frequency of gallbladder smooth muscle of guinea pig at the final concentration of 2.5×10^-4, 5×10^-4, 1×10^-3 mg·mL^-1, and these effects could be completely blocked by atropine, partially blocked by phentolamine, but not blocked by diphenhydramine and ranitidine. CONCLUSION SDT can enhance the excitability of gallbladder smooth muscle, promoting bile secretion; its choleretic effect might be mediated mainly by cholinergic M receptor, partly by epinephrine a receptor, but unrelated with histamine H1 or prostaglandin receptor.