老慢清糖浆中异秦皮啶的含量测定

Determination of Isofraxidin in Laomanqing Syrup

关键词:  高效液相色谱法  老慢清糖浆  异秦皮啶

Key words:  HPLC  Laomanqing syrup  isofraxidin

摘要:

目的 建立老慢清糖浆中异秦皮啶的含量测定方法。方法 采用反相高效液相色谱法。色谱柱：Shim-pack VP-ODS C₁₈柱(250 mm×4.6 mm，5 μm)，流动相：乙腈-0.2%磷酸(19∶81)，检测波长：344 nm，流速：1.0 mL·min^-1，柱温：30 ℃。结果 异秦皮啶在0.214~1.922 μg内线性关系良好，相关系数r=0.999 9，平均回收率为99.1%，RSD=1.2%(n=9)。 结论 该方法简便、准确、重复性好，可用于老慢清糖浆的质量控制。

Abstract:

OBJECTIVE To determine the quantity of isofraxidin in Laomanqing syrup．METHODS RP-HPLC was used．Chromatographic conditions: Shim-pack VP-ODS C₁₈ column (250 mm×4.6mm, 5 μm), the mobile phase consisted of acetonitrile-0.2% phosphoric acid aqueous solution(19∶81)，the flow rate 1.0 mL·min^-1，the detective wavelength 344 nm and column temperature 30 ℃. RESULTS The calibration curve was linear in the range of 0.214-1.922 μg for the isofraxidin(r=0.999 9)．The average recovery was 99.1% and the RSD was 1.2%(n=9)．CONCLUSION The method is simple and accurate with good repeatability, which may be used for the quality control of Laomanqing syrup.