松木层孔菌胞外多糖的组成分析及免疫学活性研究

Study on Composition and Immunomodulating Activities of Extracellular Polysaccharide from Culture Medium of Phellinus pini

关键词:  松木层孔菌  胞外多糖  组成分析  淋巴细胞增殖

Key words:  Phellinus pini  extracellular polysaccharide  composition analysis  lymphocyte transformation

摘要:

目的 研究松木层孔菌胞外多糖的单糖组成和免疫学活性。方法 使用冻融分级、酶+seveg法脱蛋白和Sepharose CL-6B层析纯化松木层孔菌胞外粗多糖(CPPE)，得到级分PPE，用HPLC分析其均一性和分子量，用气相色谱法测其单糖组成。结果 经HPLC验证，PPE为均一组分，分子量为3.9×10⁴ Da。气相色谱分析表明，PPE为甘露聚糖。淋巴细胞增殖实验显示，PPE能促进小鼠淋巴细胞的增殖，同时也可协同有丝分裂原ConA和LPS促进小鼠脾T、B淋巴细胞的增殖。结论 PPE具有提高机体免疫力的功能。

Abstract:

OBJECTIVE Study on monosaccharide composition and immunomodulating activities of extracellular polysaccharide of Phellinus pini. METHODS PPE was purified from CPPE using a combination of techniques, such as: frozen-thawed, deproteinated by enzyme/Sevag method and Sepharose CL-6B column chromatography. Homogenicity and molecular weight of PPE were analyzed by HPLC, and its composition was assayed by GC. RESULTS PPE appeared as single symmetrical peak on HPLC, and the average molecular weights was 39 kDa. Monosaccharide analysis by GC shows that it was mannan. The bioassay showed that PPE could stimulate mouse splenic lymphocytes proliferation and increase the ConA or LPS-induced lymphocytes proliferation in vitro. CONCLUSION The results showed that PPE have the function of enhancing the immunity.