依达拉奉注射液对AD大鼠学习记忆和海马内APP mRNA表达的影响

Effects of Edaravone on Learning and Memory Ability and the Mrna Expression of b-Amyloid Precursor Protein in the Hippocampus of Alzheimer’s Disease Model Rats

关键词:  尔茨海默病  依达拉奉  实验研究

Key words:  Alzheimer’s disease  edaravone  empirical study

摘要:

目的 观察依达拉奉注射液对老年性痴呆(AD)模型大鼠学习记忆功能和海马内APP mRNA表达的影响。方法 44 只大鼠随机分为空白对照组12只，造模组12只，依达拉奉低剂量干预组(干预Ⅰ组) 10只，依达拉奉高剂量干预组(干预Ⅱ组) 10只。用腹腔注射 D-半乳糖和灌胃 AlCl3法建立AD模型，从实验开始第9周开始，干预Ⅰ组和干预Ⅱ组分别于大鼠尾静脉注射依达拉奉3 mg·kg^-1·d^-1 和6 mg·kg^-1·d^-1，造模组和对照组分别注射等量生理盐水。实验开始12周后以Morris水迷宫实验检测学习记忆能力，以RT-PCR法检测大鼠脑内海马APP mRNA的表达。结果 依达拉奉能显著改善AD模型大鼠的学习记忆障碍(P<0.05)，依达拉奉干预Ⅰ组和干预Ⅱ组大鼠脑内海马内APP mRNA表达的水平亦较造模组明显降低(P<0.05)。结论 依达拉奉注射液对AD模型大鼠的学习记忆障碍有明显的改善作用。

Abstract:

OBJECTIVE To observe the effects of edaravone on learning and memory ability and the mRNA expression of β-amyloid precursor protein (APP) in the hippocampus of Alzheimer’s disease (AD) model rats. METHODS Forty-four rats　were randomly divided into 4 groups: blank control group(12), AD model group(12), edaravone low-dosage group(10) and edaravone high-dosage group(10). AD model was made by injection in peritoneum of D-galactose and administration of aluminum chloride. In the ninth week, low-dosage group and high-dosage group were injected respectively in caudal vein with edaravone 3 mg·kg^-1·d^-1 and 6 mg·kg^-1·d^-1; control group and model group were injected with equal volume normal saline. After 12 weeks, Morris water maze was used to measure the learning and memory ability, and RT-PCR was used to assay the mRNA expression of APP in the hippocampus. RESULTS Edaravone can significantly improve the disorder of learning and memory ability of AD model rats (P<0.05). The APP mRNA expression levels were significantly lower in low-dosage group and high-dosage group compared with model group (P<0.05). CONCLUSION Edaravone can significantly improve the disorder of learning and memory ability of AD model rats.