白术的UPLC指纹图谱

UPLC Fingerprint of Rhizoma Atractylodis Maerocephalae

关键词:  白术  UPLC  指纹图谱  质量评价

Key words:  Rhizoma Atractylodis Maerocephalae  UPLC  fingerprint  quality evaluation

摘要:

目的 建立白术的超高效液相色谱指纹图谱方法。方法 色谱柱为ACQUITY UPLC BEH C18(1.0 mm×50 mm，1.7 μm)，乙腈-水二元梯度洗脱模式，流速为0.1 mL·min^-1，紫外检测波长242 nm，建立了32批不同产地白术样品的UPLC指纹图谱。结果 白术药材有32个共有峰，多数峰可达到较好的分离，各批次白术药材间共有峰的相对保留时间RSD均<1.0%，药材间相似度均>90%。结论 本方法简便、可靠，较之HPLC具有更高的分辨率和灵敏度，极大地缩短了分析时间，建立的共有模式较为可靠，可用于白术药材真伪鉴别和质量评价的快速分析。

Abstract:

OBJECTIVE To establish the UPLC fingerprint analysis method for the quality evaluation of Rhizoma Atractylodis Maerocephala. METHODS UPLC fingerprint analysis method was applied for quality evaluation. ACQUITY UPLC BEH C₁₈ column was used, with the mixture of acetonitrile and water as mobile phase in a gradient mode. The flow rate was 0.1 mL·min^-1. The wavelength of measurement was 242 nm. Thirty-two batches of Rhizoma Atractylodis Maerocephalae were determined. RESULTS Most of the peaks were separated effectively, and thirty-two mutual peaks were selected from chromatography. The RSD of relative retention time of these mutual peaks was less than 1.0%, and the similarity of all samples was over 90%. CONCLUSION The method is provided with high resolving power and sensitiveness, can shorten the analyze time effectively, will be used to identify and evaluate the quality of Rhizoma Atractylodis Maerocephalae conveniently.