| 引用本文: | 沈建芳,汪红,王强,方媛.丹参中丹参酮成分的HPLC-MS(n)研究[J].中国现代应用药学,2010,27(10):944-947. |
| .Analysis of Tanshinone Constituents in Radix et Rhizoma Salviae Miltiorrhizae by HPLC-MS(n)[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(10):944-947. |
|
|
| 摘要: |
目的 研究丹参中丹参酮成分的ESI-MS(n)规律，建立其HPLC-MS(n)特征图谱。方法 以丹参酮ⅡA为代表，初步探讨丹参酮成分的ESI-MS(n)规律。应用HPLC-MS(n)，ESI(＋)模式，以水-乙腈为流动相，检测波长254 nm，梯度洗脱绘制丹参中丹参酮成分的特征图谱。结果 得出丹参酮成分的ESI-MS(n)可能的裂解途径，得到其HPLC-MS(n)特征图谱，对特征图谱中11种主要化合物进行了精确归属。结论 丹参酮类成分具有相似的ESI-MS(n)行为，根据质谱提供的各流份的分子量信息及多级质谱的裂解信息，可用于鉴别丹参酮类成分。所得到的丹参中丹参酮成分的MS(n)TIC图，可用于丹参药材中丹参酮类成分的指纹鉴别。 |
| 关键词: 丹参 丹参酮 高效液相色谱-多级质谱联用 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Analysis of Tanshinone Constituents in Radix et Rhizoma Salviae Miltiorrhizae by HPLC-MS(n) |
|
SHEN Jianfang1 WANG Hong2 WANG Qiang3 FANG Yuan1
|
| Abstract: |
| OBJCTIVE To search the ESI-MS(n) performance of tanshinone constituents in Radix et Rhizoma Salviae Miltiorrhizae and to establish its LC-MS(n) characteristic spectrums. METHODS Direct injection ESI-MS(n) has been used in the construction analysis of tanshinone ⅡA. Tanshinone constituents in Radix et Rhizoma Salviae Miltiorrhizae were studied by HPLC-MS(n). MS(n) was carried out on positive ion mode and HPLC was performed on a Zorbax SB-C18 column using gradient elution with a mixture of purified water and acetonitrile at the detection wavelength of 254 nm. RESULTS We got the proposed ESI-MS fragmention pathway of tanshinone constituents. By analyze of HPLC/ESI/MS(n) and HPLC/UV chromatograms, 11 characteristic peaks were determined. CONCLUSION Tanshinone constituents have similar ESI-MS(n) performance that can be used in the structure identification of tanshinone compounds. HPLC-MS (n) is a useful tool for a special identification of tanshinone constituents in Radix et Rhizoma Salviae Miltiorrhizae. |
| Key words: Radix et Rhizoma Salviae Miltiorrhizae tanshinone HPLC-MS(n) |
