黄芪提取物对大鼠局灶性脑缺血再灌注血脑屏障损伤的影响

Effect of Extract of Astragalus on Alterations of Blood Brain Barrier Induced by Local Cerebral Ischemia-reperfusion Injury

关键词:  黄芪提取物  局脑缺血  再灌注损伤  血脑屏障  ICAM-1  血循环内皮细胞

Key words:  extract of astragalus  local cerebral ischemia  reperfusion injury  blood-brain barrier  intercellular adhesion molecule-1  peripheral blood circulating cells

摘要:

目的 研究黄芪提取物(extract of astragalus，EA)对大鼠局灶性脑缺血(MCAO)再灌注的血脑屏障损伤的影响。方法 采用线栓法制备MCAO再灌注模型，观察EA对缺血再灌注大鼠的神经功能障碍、脑梗死体积和脑含水量、外周血循环内皮细胞(CEC)含量、缺血脑组织中细胞间粘附分子(ICAM-1)的表达、缺血侧脑组织皮质血脑屏障超微结构的病理学变化的影响。结果 EA80 mg·kg^-1，EA(40，80 mg·kg^-1)、EA(20，40，80 mg·kg^-1)可分别改善缺血再灌注2，8，24 h大鼠的神经功能障碍；能明显降低外周血中的CEC含量，明显减轻缺血脑组织的脑水肿和脑梗死体积，对脑缺血再灌注后血脑屏障超微结构的病理改变有一定的改善作用；能减少ICAM-1免疫反应阳性血管数。结论 EA对局灶性脑缺血再灌注损伤大鼠有一定的保护作用，作用机制可能与其保护血管内皮细胞，减轻脑缺血再灌注后的血脑屏障损伤有关。

Abstract:

OBJECTIVE To study the effect of extract of astragalus (EA) on alterations of blood brain barrier after local cerebral ischemia-reperfusion injury in rats. METHODS Middle cerebral artery occlusion (MCAO) was used to make local cerebral ischemia-reperfusion model with intravascular nylon filament occlusion. The neurological function score, brain edema and infarct volume, the counts of peripheral blood circulating cells, the expression of intercellular adhesion molecule-1, pathological changes in blood-brain barrier ultrastructure in rats after focal cerebral ischemia-reperfusion were observed. RESULTS EA 80 mg·kg^-1 decreased the neurological function score; EA(40 and 80 mg·kg^-1) reduced brain edema and infarct volume, the counts of peripheral blood circulating cells, pathological changes in blood-brain barrier ultrastructure; EA(20, 40 and 80 mg·kg^-1) decreased the expression of intercellular adhesion molecule-1 in brain tissue. CONCLUSION EA has protective effects against cerebral ischemia reperfusion injuries, the mechanism may be related with reducing BBB injury induced by local cerebral ischemia-reperfusion.