北豆根脂肪油提取工艺优化及其抗氧化活性研究

Study on Extraction Process and Antioxidant Activity of Menispermi Rhizoma Fatty Oil

关键词:  北豆根脂肪油  Box-Behnken响应面法  气相色谱-质谱联用法  体外抗氧化  1,1-二苯基-2-三硝基苯肼自由基

Key words:  Menispermi Rhizoma fatty oil  Box-Behnken response surface methodology  GC-MS  antioxidant activity in vitro  DPPH free radical

摘要:

目的 采用Box-Behnken响应面法优化北豆根脂肪油的提取工艺，分析其成分组成，并研究北豆根脂肪油的体外抗氧化活性。方法 以北豆根脂肪油提取率为指标，在单因素试验基础上，采用Box-Behnken响应面法考察提取温度、提取时间、料液比对提取工艺的影响；采用气相色谱-质谱联用法（GC-MS）分析脂肪油的组成；运用清除1，1-二苯基-2-三硝基苯肼（1，1-diphenyl-2-picrylhydrazyl，DPPH）自由基试验评价北豆根脂肪油的体外抗氧化活性。结果 经响应面优化，确定最佳提取工艺为提取温度90℃，回流提取3次，每次2.3 h，料液比为1：21。在上述条件下，提取率为1.820%；采用GC-MS从北豆根脂肪油中鉴定出32个成分；北豆根脂肪油总抗氧化活性随着浓度的增加而逐渐升高，在浓度达到0.833 mg·mL^-1后对DPPH自由基清除率的增幅变缓，当浓度为1.333 mg·mL^-1时，清除率为70.1%。结论 优化的工艺方便、稳定、可行，可为后续研究提供参考，北豆根脂肪油中含有多种重要脂肪油成分，并具有一定的体外抗氧化能力。

Abstract:

OBJECTIVE To optimize the extraction process of Menispermi Rhizoma fatty oil by Box-Behnken response surface methodology and analyze its composition and study the antioxidant activity of Menispermi Rhizoma fatty oil in vitro. METHODS The extraction rate of Menispermi Rhizoma fatty oil as an index and based on the single-factor tests, Box-Behnken response surface methodology was used to investigate the effects of extraction temperature, extraction time and material-liquid ratio on extraction process; the composition of fatty oil was analyzed by GC-MS; the 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical experiment was used to evaluate the in vitro antioxidant activity of Menispermi Rhizoma fatty oil. RESULTS After optimization with the response surface methodology, it was determined that the optimal extraction process was extraction temperature of 90℃ and reflux extraction of 3 times for 2.3 h each time, the ratio of solid to liquid was 1:21. Under these conditions, the extraction rate was 1.820%; there were 32 components identified from Menispermi Rhizoma fatty oil by GC-MS. The total antioxidant activity of Menispermi Rhizoma fatty oil increased gradually with the increase of the concentration, after the concentration reached 0.833 mg·mL^-1, the DPPH free radical scavenging rate increased slowly, at the concentration of 1.333 mg·mL^-1, the scavenging rate was 70.1%. CONCLUSION The optimized process is convenient, stable and feasible, which can provide references for subsequent research. Menispermi Rhizoma fatty oil contains many important fatty oil components and has certain antioxidation activity in vitro.