血必净对脓毒症肝损伤大鼠氧化应激及炎症状态的影响

Effects of Xuebijing Injection on Oxidative Stress and Early Inflammatory Factors in Rats with Sepsis Induced Liver Injury

关键词:  血必净  脓毒症  肝损伤  氧化应激  炎症因子

Key words:  Xuebijing injection  sepsis  hepatic injury  oxidative stress  inflammation factor

摘要:

目的 观察早期应用血必净注射液对脓毒症大鼠急性肝损伤的抗氧化应激保护作用和对炎症因子的抑制作用。方法 54只健康Wistar大鼠随机分为对照组、脓毒症组和血必净组，盲肠结扎穿孔法制造脓毒症模型。各组在建立模型后6，12，24 h作为观察点，观察脓毒症肝损伤的肝脏氧化应激反应、细胞炎症因子的变化和肝细胞的病理改变。结果 盲肠结扎穿孔后6，12 h与对照组比较，脓毒症组、血必净组的氧化应激指标尚无明显变化，而CRP出现明显升高（P<0.05）；12 h后与脓毒症组比较，血必净组的MDA、TNF-α下降、IL-10开始升高；24 h后与脓毒症组相比，血必净组血清ALT，AST降低（P<0.05），肝脏GSH、SOD含量明显升高（P<0.05），MDA含量降低（P<0.05），血清CRP、TNF-α浓度明显降低（P<0.01），而IL-10明显升高（P<0.01）。病理分析表明12，24 h血必净组在细胞形态学上较脓毒症组有明显改善（P<0.05，P<0.01）。结论 脓毒症早期应用血必净注射液能抗氧化应激反应、稳定肝细胞膜通透性和对炎症因子的抑制作用，对脓毒症导致的急性肝脏损伤起到一定的保护作用。

Abstract:

OBJECTIVE To observe the effect of Xuebijing injection in rats with sepsis induced liver injury and explain the mechanism from anti-oxidative stress and early inflammatory factors. METHODS Fifty-four healthy Wistar rats were randomly divided into control group, sepsis group and Xuebijing group. The rat model of sepsis was established with cecal ligation puncture (CLP). Serum samples and indices of liver tissue were measured at the point of 6, 12, 24 h after model was established. Observed the effect of Xuebijing injection on liver anti-oxidation, hapatocyte membrance permeability, and change of inflammatory cytokines. RESULTS Compared with control gruop, the concentrations of peroxidation in sepsis group and Xuebijing group were no significant difference after 6, 12 h of established rat model, however C-react protein(CRP) was much higher. After 12 h of CLP, MDA and TNF-α decreased and IL-10 began to increase in Xuebijing group compared with sepsis group. But ALT and AST in Xuebijing group was lower (P<0.05) than sepsis group after 24 h. The expression of GSH and SOD in livers of Xuebijing group were significantly higher (P<0.05), and MDA lower than sepsis group(P<0.05). Meanwhile, CRP, serum tumor TNF-α was decreased (P<0.01) and interleukin-10(IL-10) increased in Xuebijing group compared to sepsis group. According to liver histopathological changes, Xuebijing group showed better pathologic changes compaired to sepsis group after 12, 24 h(P < 0.05, P < 0.01). CONCLUSION Xuebijing injection show protective effect on acute liver injury of sepsis. Its mechanism may be correlated with anti-oxidative stress of liver, protection of hapatocyte membrance permeability and inhibition of some inflammatory factors.