全反式维甲酸对小鼠神经系统作用的一般药理试验研究

General Study on the Effect of All-trans-retinoic Acid on the Nervous System of Mice

关键词:  自主活动实验  协调运动实验  全反式维甲酸

Key words:  spontaneous activity test  coordination test  all-trans-retinoic acid

摘要:

目的 通过小鼠自主活动实验、小鼠协调运动实验、与阈下剂量戊巴比妥钠协同作用实验考察全反式维甲酸（all-trans-retinoic acid，ATRA）对小鼠神经系统的影响。方法 ICR小鼠，♀♂各半，按体质量随机分为溶剂对照组、250.0，50.0，10.0 mg·kg^-1 ATRA组，各组小鼠禁食（不禁水）12 h后分别灌胃给予相应剂量ATRA，给药体积为0.02 mL·g^-1。小鼠自主活动实验，测定给药前及给药后6，120，180 min小鼠在5 min内的活动次数。小鼠协调运动试验，测定给药后120 min小鼠在转棒上的停留时间。与阈下剂量戊巴比妥钠协同作用试验，给药后120 min腹腔注射戊巴比妥钠阈下剂量，观察睡眠小鼠的例数。结果 ATRA 250.0 mg·kg^-1作用120 min后，小鼠自主活动次数显著减少，给予50.0，10.0 mg·kg^-1 ATRA 120，180 min后均可见小鼠自主活动减少的趋势；而250.0，50.0及10.0 mg·kg^-1 ATRA对小鼠的协调运动均无明显影响，与阈下剂量的戊巴比妥钠无协同作用。结论 ATRA能减少小鼠自主活动次数，对协调运动无明显影响，与阈下剂量戊巴比妥钠无协同作用。

Abstract:

OBJECTIVE To investigate the effect of the all-trans-retinoic acid(ATRA) on the nervous system of mice by employment mice spontaneous activity test, coordination test, and the synergistic effect of the threshold dose of sodium pentobarbital test. METHODS ICR mice, half male and half female, were randomly assigned as vehicle, 250.0, 50.0 and 10.0 mg·kg^-1 ATRA groups according to body weight. After 12 h of fasting (with water), the mice in each group were given the corresponding dose of ATRA respectively by intragastrical administration, dosing volume was 0.02 mL·g^-1. As to mice spontaneous activity test, the mice spontaneous activity was determined before and after administration of ATRA for 60, 120, and 180 min. Mice coordinated test was carried out by determining the residence time of mice on the rotary rod 120 min after administration. The synergistic effect of the threshold dose of sodium pentobarbital and ATRA was analyzed 120 min after administration of sodium pentobarbital. The numbers of sleeping mice were calculated. RESULTS The 250.0 mg·kg^-1 ATRA could significantly reduce the number of spontaneous activity of mice, 50.0 or 10.0 mg·kg^-1 ATRA was found to reduce the trend of spontaneous activities in 120, 180 min; 250.0, 50.0, or 10.0 mg·kg^-1 ATRA had no significant effect on the coordination of mice. ATRA had no synergistic effect with the threshold dose of sodium pentobarbital. CONCLUSION ATRA can reduce the number of spontaneous activity of mice, and has no significant effect on the coordination of mice, and has no synergistic effect with the threshold dose of sodium pentobarbital.