山慈菇HPLC指纹图谱质量评价

Study on HPLC Fingerprints of Cremastrae Pseudobulbus Pleiones Pseudobulbus

关键词:  山慈菇(冰球子)  指纹图谱  质量评价  高效液相色谱法

Key words:  Cremastrae Pseudobulbus Pleiones Pseudobulbus  fingerprints  quality evaluation  HPLC

摘要:

目的 建立山慈菇（冰球子）的HPLC指纹图谱，为山慈菇（冰球子）药材的质量评价提供科学依据。方法 采用Agilent 1200 Topsil^TM（拓谱）C₁₈色谱柱（4.6 mm×250 mm，5 μm），乙腈和水为流动相，梯度洗脱，流速为1.0 mL·min^-1，检测波长为224 nm，柱温为30℃。结果 10批山慈菇（冰球子）药材HPLC指纹图谱共检出7个分离度良好的共有指纹峰。样品中参照物1,4-二[4-（葡萄糖氧）苄基]-2-异丁基苹果酸酯的含量有明显差别。结论 该法简便、快速，可作为山慈菇（冰球子）的质量评价的有力手段。

Abstract:

OBJECTIVE To establish the HPLC fingerprints of chemical constituents in Cremastrae Pseudobulbus Pleiones Pseudobulbus and provide the basis for the quality evaluate of it. METHODS The chromatographic separation was performed on an Agilent 1200 Topsil^TM C₁₈(4.6 mm×250 mm, 5 μm) column, and the mobile phase was consisted of acetonitrile and water with gradient elution. The flow rate was 1.0 mL·min^-1, the detection wavelength was 224 nm, and the column temperature was 30℃. RESULTS There were 7 common peaks in the HPLC fingerprints of 10 batches of Cremastrae Pseudobulbus Pleiones Pseudobulbus, and the 7 characteristic peaks showed obvious difference in Cremastrae Pseudobulbus Pleiones Pseudobulbus from different habitats. Significant difference was observed in the militarine content among the samples. CONCLUSION The HPLC fingerprints method is simple, rapid and feasible in the quality control of Cremastrae Pseudobulbus Pleiones Pseudobulbus.