| 引用本文: | 袁子民,高春华,王静,李学涛.β-榄香烯聚合物胶束的制备工艺研究[J].中国现代应用药学,2016,33(10):1280-1283. |
| YUAN Zimin,GAO Chunhua,WANG Jing,LI Xuetao.Preparation of β-elemene-loaded Nano Polymeric Micelles[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(10):1280-1283. |
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| 摘要: |
| 目的 优化β-榄香烯纳米聚合物胶束的制备工艺。方法 以二硬脂酰磷脂酰乙醇胺聚-乙二醇（DSPE-PEG2000）为纳米聚合物胶束的载体材料，采用薄膜水化法，以包封率为考察指标，通过正交试验考察β-榄香烯与DSPE-PEG2000的质量比、水化时间、水化体积、PBS缓冲液的pH值对制备工艺的影响。结果 在β-ELE与DSPE-PEG2000的质量比为1：10，水化体积为5 mL，水化时间为30 min，所用PBS缓冲液的pH值为7.4时，所制得的纳米聚合物胶束形态规则，大小较为均匀，平均粒径为56 nm，平均包封率为88.83%。结论 该制备工艺稳定可行，重复性好，可用于β-榄香烯纳米聚合物胶束的制备。 |
| 关键词: β-榄香烯 纳米聚合物胶束 薄膜水化法 |
| DOI：10.13748/j.cnki.issn1007-7693.2016.10.014 |
| 分类号: |
| 基金项目:辽宁省科学技术计划项目（2015020703） |
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| Preparation of β-elemene-loaded Nano Polymeric Micelles |
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YUAN Zimin1, GAO Chunhua2, WANG Jing1, LI Xuetao1
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1.School of Pharmacy, Liaoning University of TCM, Dalian 116600, China;2.School of Pharmacy, Jinzhou Medical University, Jinzhou 121001, China
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| Abstract: |
| OBJECTIVE To optimize the preparation technology of β-elemene-loaded nano polymeric micelles. METHODS With poly(ethylene glycol-2000)-grafted distearoy lphosphosphatidylethanolamine(DSPE-PEG2000) as the carrier materials, thin film hydration method was used to prepare β-elemene-loaded nano polymeric micelles; With entrapment efficiency of β-elemene as index, orthogonal design was adopted to optimize effects of the ratio of β-elemene and DSPE-PEG2000, volumn and time of hydration, pH of PBS. RESULTS In the optimal conditions, the mass ratio of β-elemene and DSPE-PEG2000 of 1:10, hydration volume of 5 mL, hydration time of 30 min, PBS buffer pH of 7.4, the prepared nanoparticles were round and regular in shape without adhesions, the average of particle size was 56 nm, the average of entrapment efficiency was 88.83%. CONCLUSION The preparation established in this optimization method is stable and feasible, and it could be used for the preparation of β-elemene-loaded nano polymeric micelles. |
| Key words: β-elemene nano polymeric micelles thin film hydration method |
