氧氟沙星/蒙脱土纳米复合体的制备及其对药物缓释作用的研究

Study on Preparation and Sustained Release Behavior of the OFLO/MMT Nanocomposite

关键词:  蒙脱土  纳米复合物  氧氟沙星  体外释放

Key words:  montmorillonite  nanocomposite  ofloxacin  in vitro release

摘要:

目的 制备氧氟沙星/蒙脱土（OFLO/MMT）插层化复合物，为寻求新型药物载体材料及氧氟沙星新剂型提供依据。方法 采用溶液离子交换法制备OFLO/MMT复合体系，利用单因素法考察药物浓度、反应温度、时间等因素对蒙脱土载药量的影响，优化插层反应条件，通过紫外光谱、傅立叶变换红外（FTIR）、DTA曲线手段对插层复合体进行表征。并进一步研究OFLO/MMT纳米复合物在不同pH值释放介质中的释放行为。结果 在初始浓度2.60 mg·mL^-1，25℃反应2 h时药物载药量最大，体外释放实验表明OFLO/MMT插层药物在释放介质中具有缓释作用，且OFLO/MMT在酸性和碱性条件下的释放动力学明显不同，复合体更倾向于在盐酸溶液（pH 1.2）中进行释放。结论 插入蒙脱土层间的氧氟沙星具有明显的缓释作用，且具有一定pH值敏感性，蒙脱土可作为一种非常好的缓释制剂载体材料。

Abstract:

OBJECTIVE The OFLO/MMT nanocomposite was prepared to find the new drug delivery material and develop the new dosage of ofloxacin. METHODS The OFLO/MMT nanocomposite was prepared by the solution ion exchange and the factors such as drug initial concentration, reaction time and the reaction temperature on the drug loading of the nanocomposite were studied by the single factor method and the intercalation reaction condition was optimized. The structure of the nanocomposite was characterized by Ultraviolet spectrum, FTIR and DTA. Moreover the in vitro release behaviors of the OFLO/MMT in different pH medium were investigated. RESULTS The optimal conditions of the intercalation reaction were as follows:the initial concentration was 2.60 mg·mL^-1, the temperature was 25℃ and the time was 2 h. The sustained release behavior was obviously observed in vitro release experiment for the OFLO/MMT composite, and the release kinetic behavior in acid medium was different from that of basic medium. Generally, it was more easily for the OFLO/MMT release in acid solution (pH 1.2). CONCLUSION The ofloxacin intercalated into the layer of the montmorrilloniite releases slowly from the composite, the montmorrillonite has a great potential as a delivery host in the pharmaceutical field.