| 引用本文: | 严莹莹,王曼莉,李锦鸿,李承龙,洪光博,黄丽平.石菖蒲中β-细辛醚的提取工艺优化及其体外抗氧化活性研究[J].中国现代应用药学,2024,41(1):18-26. |
| YAN Yingying,WANG Manli,LI Jinhong,LI Chenglong,HONG Guangbo,HUANG Liping.Optimization of Extraction Technology and Antioxidant Activity of β-Asarone from Acori Tatarinowii Rhizoma in Vitro[J].Chin J Mod Appl Pharm(中国现代应用药学),2024,41(1):18-26. |
|
| 摘要: |
| 目的 采用乙醇加热回流法研究石菖蒲β-细辛醚的最佳提取工艺，并考察其不同部位抗氧化活性。方法 以石菖蒲β-细辛醚提取量为评价指标，优化提取方法，在单因素试验的基础上，采用正交设计与响应面法考察乙醇浓度、料液比、提取时间3个因素对石菖蒲β-细辛醚提取量的影响。在确定最佳提取工艺后，对其不同部位抗氧化活性进行研究。结果 石菖蒲β-细辛醚最佳提取工艺：乙醇浓度为95%，料液比为1∶20 g·mL–1，提取时间为2.5 h。在此条件下，石菖蒲β-细辛醚提取量为0.918 7 mg·g–1。体外抗氧化活性结果表明抗氧化能力强弱顺序依次为乙酸乙酯>石油醚>乙醇>正丁醇。其中，乙酸乙酯部位抗氧化活性最强，具有较好清除DPPH、ABTS自由基的能力，同时具备一定的还原能力。结论 优选工艺方法简单、稳定可靠，可用于石菖蒲β-细辛醚的提取和抗氧化活性测定，为石菖蒲的进一步开发提供依据。 |
| 关键词: 石菖蒲 β-细辛醚 抗氧化 正交试验 响应面 |
| DOI：10.13748/j.cnki.issn1007-7693.20222806 |
| 分类号:R285.5 |
| 基金项目:国家自然科学基金项目(31900297)；燕岭优秀青年教师培养计划项目(YL20200210)；岭南师范学院红树林研究院开放课题资助(YBXM01)；粤西特色生物医药工程技术研究中心开放课题(2022K03) |
|
| Optimization of Extraction Technology and Antioxidant Activity of β-Asarone from Acori Tatarinowii Rhizoma in Vitro |
|
YAN Yingying1, WANG Manli1, LI Jinhong1, LI Chenglong1, HONG Guangbo1, HUANG Liping1,2
|
|
1.Lingnan Normal University, Mangrove Institute, Zhanjiang 524048, China;2.Lingnan Normal University, Western Guangdong Characteristic Biology and Medicine Englineering and Research Center, Zhanjiang 524048, China
|
| Abstract: |
| OBJECTIVE To study the best extraction process of β-asarone from Acori Tatarinowii Rhizoma by ethanol heating reflux method, and to explore the antioxidant activity of different segments. METHODS With β-asarone from Acori Tatarinowii Rhizoma as the evaluation index to optimize the extraction method. On the basis of a single factor experiment, the effects of ethanol concentration, solid-liquid ratio and extraction time on the extraction amount of β-asarone from Acori Tatarinowii Rhizoma were investigated by orthogonal design and response surface methodology. After the optimal extraction process was determined, the antioxidant activities of different segments were studied. RESULTS The optimum extraction process of β-asarone from Acori Tatarinowii Rhizoma was as follows: ethanol concentration was 95%, solid-liquid ratio was 1∶20 g·mL–1 and extraction time was 2.5 h. Under these conditions, the extraction amount of β-asarone from Acori Tatarinowii Rhizoma was 0.918 7 mg·g–1. The results of in vitro antioxidant activity showed that the order of antioxidant capacity was ethyl acetate>petroleum ether>ethanol>n-butanol. Among them, the ethyl acetate fraction had the strongest antioxidant activity, with good ability to scavenge DPPH and ABTS free radicals, and had certain reduction ability. CONCLUSION The optimized method is stable, reliable and simple, which can be used for extraction and antioxidant activity determination of β-asarone from Acori Tatarinowii Rhizoma, and provides a basis for the further development of Acori Tatarinowii Rhizoma. |
| Key words: Acori Tatarinowii Rhizoma β-asarone antioxidant orthogonal test response surface |
