甘肃栽培蒙古黄芪横切面组织显微定量与质量标志物含量的相关性研究

Study on the Correlation Between the Microstructure Quantification and the Content of Quality Markers of Cultivated Astragalus Membranaceus(Fisch.)Bge.var.Mongholicus(Bge.)Hsiao in Gansu Province

关键词:  蒙古黄芪  组织显微定量  质量标志物  相关性  商品等级划分  质量评价

Key words:  Astragalus membranaceus(Fisch.)Bge. var. mongholicus(Bge.)Hsiao  microstructure quantification  quality markers  relevance  commodity grade division  quality evaluation.

摘要:

目的 研究蒙古黄芪横切面组织显微定量与质量标志物含量的相关性，为蒙古黄芪商品等级划分和质量评价提供依据。方法 测量13批蒙古黄芪药材横切面7个组织显微定量参数，HPLC测量质量标志物中7个化学成分；对13批蒙古黄芪药材的7个组织显微定量参数和7个质量标志物及其相加相除运算的8个结果(以下简称“运算结果”)，分别运用SPSS 26.0软件进行Spearman相关性分析和聚类分析，Graphpad Prism 8.0对显著相关变量进行多元线性回归，SIMCA 14.1进行主成分分析。结果 木射线数、木质部宽度、木栓层宽度、韧皮部宽度/木质部宽度与质量标志物及其运算结果存在多对显著相关关系，蒙古黄芪横切面组织形态可以描述质量标志物含量。主成分分析提取了2个主成分，对主成分贡献较大的6个变量分别是毛蕊异黄酮苷、芒柄花苷、韧皮部宽度/木质部宽度、黄芪甲苷、黄芪皂苷I、黄芪皂苷II，其将13批蒙古黄芪药材分为2类，结果与聚类分析一致。结论 本研究基于横切面组织显微定量与质量标志物含量的相关性，以蒙古黄芪横切面组织显微定量参数间接表征质量标志物的含量，为蒙古黄芪药材商品等级划分的综合依据提供新的科学基础，对于蒙古黄芪药材质量现代化评价模式的形成具有参考价值。

Abstract:

OBJECTIVE To provide the basis for commodity grade classification and quality evaluation of Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao by studying the correlation between the microscopic quantification of transsection tissue of Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao and the content of quality markers. METHODS The microscopical quantitative parameters of 7 tissues in the cross section of 13 batches of Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao were measured, and the chemical components of 7 quality markers were measured by HPLC. For 7 tissues microquantitative parameters and 7 quality markers of 13 batches of Astragalus membranaceus (Fisch.)Bge.var.mongholicus(Bge.)Hsiao and 8 results of addition and division calculation(hereinafter referred to as “calculation results”), SPSS 26.0 software was used for Spearman correlation analysis and cluster analysis, Graphpad Prism 8.0 was used for multiple linear regression of significant correlation variables, and SIMCA 14.1 was used for principal component analysis. RESULTS There were many pairs of significant correlation between wood ray number, xylem width, cork layer width, phloem width/xylem width and the quality markers and their calculation results. The tissue morphology of Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao transverse section could describe the content of quality markers. Two principal components were extracted by principal component analysis, and the six variables that contributed significantly to the principal components were calycosin-7-glucoside, ononin, phloem width/xyloside width, astragaloside IV, Astragaloside I and Astragaloside II. Thirteen batches of Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao were divided into two categories, and the results were consistent with cluster analysis. CONCLUSION Based on the correlation between the microscopic quantification of cross-section tissue and the content of quality markers, this study indirectly represents the content of quality markers with the microscopic quantitative parameters of Astragalus membranaceus(Fisch.)Bge. var. mongholicus(Bge.) Hsiao cross-section tissue, which provides a new scientific basis for the comprehensive basis of commodity grade classification of Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao medicinal materials, and has reference value for the formation of modern evaluation model of Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao medicinal materials quality.