细胞膜红色荧光探针PLGA纳米粒的制备及表征

Preparation and Characterization of DiI Loaded PLGA Nanoparticles

关键词:  细胞膜红色荧光探针  聚乳酸-羟基乙酸共聚物纳米粒  单甲氧基聚乙二醇聚乳酸-羟基乙酸共聚物纳米粒  自乳化溶剂扩散法  超滤法  紫外可见分光光度法

Key words:  fluorescent probe(DiI)  PLGA nanoparticles  MePEG-PLGA nanoparticles  spontaneous emulsion solvent diffusion method  ultrafiltration  UV-visible spectrophotometry

摘要:

目的 以聚乳酸-羟基乙酸共聚物(PLGA)和单甲氧基聚乙二醇聚乳酸-羟基乙酸共聚物(MePEG-PLGA)为材料，制备包载细胞膜红色荧光探针(DiI)的纳米粒，为后续的细胞实验奠定基础。方法 采用自乳化溶剂扩散法制备纳米粒；超滤法分离纯化纳米粒，紫外可见分光光度法测定DiI的含量，并计算包封率。结果 DiI用量为0.5 mg，PLGA或MePEG- PLGA投入量为50 mg，PVA浓度为0.5%，可制得粒径较合适的纳米粒。DiI-PLGA-NP平均粒径为(280.7±3.6)nm，Zeta电位为(-2.98±0.47)mV，包封率可达88.0%； DiI-MePEG-PLGA-NP平均粒径为(157.2±3.2)nm，Zeta电位为(-4.90±0.54)mV，包封率可达87.1%。结论 DiI作为脂溶性良好的示踪剂，用其制得的荧光素纳米粒可为后续的体外实验奠定良好的基础。

Abstract:

OBJECTIVE To prepare DiI loaded nanoparticles using PLGA and MePEG-PLGA as carriers. METHODS Spontaneous emulsion solvent diffusion method(SESD) was adopt to prepare nanoparticles. Ultrafiltration-UV-visible spectrophotometry was used for the determination of DiI-NP' encapsulation efficiency. RESULTS When DiI’s dosage was 0.5 mg, PLGA’s or MePEG-PLGA’s dosage was 50 mg, PVA’s content was 0.5%, the suitable nanoparticles were obtained. DiI-PLGA-NP’ average diameter was (280.7±3.6)nm, DiI-PLGA-NP’ Zeta potential was (-2.98±0.47)mV, DiI-PLGA-NP’ encapsulation efficiency was 88.0%; DiI-MePEG-PLGA-NP’ average diameter was (157.2±3.2)nm, DiI-MePEG-PLGA-NP’ Zeta potential was (-4.90±0.54)mV, DiI-MePEG-PLGA-NP’ encapsulation efficiency was 87.1%. CONCLUSION As a liposoluble long-term tracer, DiI-NP can lay the foundation for subsequent in vitro tests.