青蒿琥酯对人结肠腺癌细胞的放射增敏作用

Radiosensitizing Effect of Artesunate on Human Colon Adenocarcinoma

关键词:  青蒿琥酯  结肠腺癌  放射增敏

Key words:  artesunate  colon adenocarcinoma  radiosensitization

摘要:

目的 研究青蒿琥酯对人结肠腺癌SW480细胞的放射增敏作用。方法 用MTT法测定青蒿琥酯的半数抑制浓度(IC₅₀)。将SW480细胞分为4组：对照组、药物组(给予20% IC₅₀的青蒿琥酯)、照射组(给予6 MV 4 Gy X射线照射)、联合组(经20% IC₅₀浓度的青蒿琥酯培养8 h后，给予6 MV 4 Gy X射线照射)。MTT法测定4组细胞增殖抑制率。再测定细胞集落形成情况，计算细胞集落形成率(PE)，细胞存活分数(SF)。根据多靶单击公式，计算平均致死剂量、外推值、2 Gy时的细胞存活分数和放射增敏比。用流式细胞仪测定各组细胞的凋亡率。结果 青蒿琥酯的IC50为20.87 μg·mL^-1。与对照组相比，3个实验组的细胞增殖抑制率和凋亡率有显著差异(P<0.05)，而与其他3组相比，联合组的细胞增殖抑制率和凋亡率有显著差异(P<0.05)。放射增敏比为1.38。结论 青蒿琥酯对人结肠癌腺癌SW480细胞有一定的放射增敏作用，机制可能与促进凋亡有关。

Abstract:

OBJECTIVE To study the radiosensitizing effect of artesunate on human colon adenocarcinoma cells SW480. METHODS IC₅₀ of artesunate was measured by MTT. SW480 cells were divided into 4 groups: control group, drug group(artesunate of 20% IC₅₀), radio group(radiation of 6 MV X ray), and combined group(received artesunate of 20% IC₅₀ and radiation of 6 MV X ray). MTT was used to measure inhibitory rate of cell proliferation. Then the formation of cell colony was detected. Colony forming rate (PE) and survival fraction (SF) were calculated. Based on multiple targeting simple hit formula, mean lethal dose, extrapolation value, cell survival fraction and radiosensitization ratio of 2 Gy were calculated. The apoptosis rate was measured by flow cytometry. RESULTS Compared with control group, there were significant differences in inhibitory rate of cell proliferation and apoptosis rate of three groups(P<0.05). Compared with other three groups, there was significant difference in inhibitory rate of cell proliferation and apoptosis rate of combined group(P<0.05). Radiosensitization ratio was 1.38. CONCLUSION Artesunate has radiosensitizing effect on human colon adenocarcinoma cells of SW480, and the mechanism might be related to promoting apoptosis.