白花蛇舌草栽培品与野生品有效成分含量测定

Determination of the Content of Effective Component of Wild and Cultivated Products of Hedyotis Diffusa

关键词:  白花蛇舌草  齐墩果酸  熊果酸  总黄酮  野生品  栽培品  高效液相色谱法

Key words:  Hedyotis diffusa  oleanolic acid  ursolic acid  total flavonoids  wild products  cultivated products  HPLC

摘要:

目的 测定白花蛇舌草野生品和栽培品有效成分的含量，为白花蛇舌草的质量标准和规范化栽培提供依据。方法 采用HPLC测定白花蛇舌草中齐墩果酸和熊果酸的含量，采用紫外分光光度法测定总黄酮的含量。结果 不同产地白花蛇舌草野生品中3种成分含量差异较大，其中齐墩果酸含量为0.328 1~1.191 7 mg·g^-1，熊果酸含量为1.686 5~2.641 0 mg·g^-1，总黄酮含量为0.921 9%~1.611 7%；广东、广西栽培品中3种成分含量均较高，且不同批次栽培品中各成分含量没有显著差异，具有一定的稳定性。结论 白花蛇舌草由野生转化为家种，有利于保证药材的有效性和稳定性。

Abstract:

OBJECTIVE To determine the content of the active component of wild and cultivated products of Hedyotis diffusa, provide a basis for its quality standards and standardized cultivation. METHODS The content of oleanolic acid and ursolic acid in Hedyotis diffusa was determined by HPLC, and the content of total flavonoids was determined by UV. RESULTS The content of three kinds of active component were significantly different in different areas of wild products, and the content of oleanolic acid was 0.328 1-1.191 7 mg·g^-1; the content of ursolic acid was 1.686 5-2.641 0 mg·g^-1; the content of total flavonoids was 0.921 9%-1.611 7%. The content in the three kinds of Guangdong and Guangxi cultivated products were higher, there was no significantly difference between them, and kept certain stability. CONCLUSION Application of the home-grown product instead of wild products of Hedyotis diffusa, can ensure the effectiveness and stability of the quality.