消瘤片对小鼠子宫内膜异位症和抗炎、镇痛作用研究

Effect of Xiaoliu Tablet on Mice with Experimental Endometriosis, Anti-inflammatory and Analgesia

关键词:  消瘤片  子宫内膜异位症  抗炎  镇痛

Key words:  Xiaoliu tablet  endometriosis  anti-inflammatory  analgesic

摘要:

目的 观察消瘤片对实验性子宫内膜异位症小鼠异位内膜、免疫功能的影响和抗炎、镇痛的作用。方法 进行小鼠子宫内膜移植术，观察消瘤片对子宫内膜异位症小鼠异位病灶和免疫功能的作用；采用二甲苯致小鼠耳廓肿胀、冰醋酸致小鼠腹腔毛细血管通透性增加，观察消瘤片对急性炎症的作用；镇痛实验采用醋酸扭体法和鼠尾热刺痛法观察消瘤片对疼痛的影响。结果 与模型组相比，消瘤片可以减轻异位病灶黏连程度、面积与体积，其中高剂量组可以显著减小异位病灶的体积(P<0.05)；消瘤片可以促进子宫内膜异位症小鼠脾脏系数的降低和促进胸腺系数的上升，但统计均没有显著性差异。消瘤片高剂量组可明显抑制二甲苯致的小鼠耳廓肿胀及醋酸所致的小鼠腹腔毛细血管通透性增高；还可以显著抑制醋酸诱发的扭体反应，并显著延长60 min时的甩尾潜伏期(P<0.05)。结论 消瘤片可以抑制子宫内膜异位症模型小鼠异位内膜的黏附和病灶的扩大，高剂量组具有较强的抗炎、镇痛作用。

Abstract:

OBJECTIVE To observe the effects of Xiaoliu tablet(XLT) on ectopic endometrial, immunity, anti-inflammation and analgesic in mice with endometriosis. METHODS The effect of XLT on immunologic function in mice was studied by transplantation of endometrium. The role of XLT on acute inflammation with xylene-induced mouse ear oedema and abdominal capillary permeability increase caused by glacial acetic acid in mice was observed. Analyesia experiments adopted acetic acid-induced writhing and tail-flick test. RESULTS XLT could reduce the degree of adhesion, area and volume on ectopic focal. Compared with model group, the high dose of XLT group could significantly reduce the volume of ectopic lesions(P<0.05). XLT could reduce the spleen coefficient and promote rising on thymus coefficient, but there were no significant difference (P>0.05). High dose of XLT group obviously inhibited the swelling of mouse pinna induced by xylene, suppressed the increase of mouse blood capillary permeability by acetic acid, significantly depressed the torsion boby of mouse and increased the tail flick latency of 60 minutes(P<0.05). CONCLUSIONS XLT can inhibit adhesion of ectopic endometrial and the enlargement of lesions on endometriosis, high dose group has significant anti-inflammatory and analgesic effect in mice models.