逆流色谱中多种洗脱模式的应用研究进展

Progress of Different Elution Modes of Counter-current Chromatography

关键词:  逆流色谱  洗脱模式  基本原理  进展

Key words:  counter-current chromatography  elution mode  fundamental principle  progress

摘要:

目的 介绍现代逆流色谱中多种洗脱模式的应用研究进展，为分析工作者提供相关研究思路。方法 通过综述国内外相关文献、著作，对比不同洗脱模式，得出各洗脱模式适用范围及其优缺点。结果 总结了包括梯度洗脱模式、双向洗脱模式、多重双向洗脱模式、闭路循环洗脱模式、洗脱-推挤模式、洗脱-反推模式和pH区带精制模式等多种逆流色谱洗脱模式的基本原理及其近年来的研究和应用进展。结论 逆流色谱不同洗脱模式、不同分析分离手段各有着自身的优缺点，在逆流色谱分离分析中应根据被分离溶质特性选择适当的洗脱模式以达到最佳分离效果。

Abstract:

OBJECTIVE To review the recent progress on various kinds of elution modes emerged during the modern counter-current chromatographic techniques in detail. METHODS Almost all the related literatures about studying on different kinds of elution modes for counter-current chromatography were summarized, in which its applications, advantages and disadvantages of each elution mode were in introduced. RESULTS Totally seven types of elution modes that frequently used in the modern counter-current chromatography were described, including gradient elution mode, dual-mode elution, multiple-dual mode, closed-loop recycling mode, elution-extrusion mode, elution-back extrusion mode and pH-zone-refining mode. CONCLUSION Each of elution mode for counter-current chromatography owns its distinctive characteristic and should be selected in light of analytes be separated by this technique, in order to achieve better separations.