大孔吸附树脂富集参血胶囊中人参皂苷Rb1的工艺研究

Study on Enrichment of Ginsenoside Rb1 in Shenxue Capsules by Macroporous Adsorptive Resin

关键词:  参血胶囊  人参皂苷Rb₁  大孔吸附树脂  富集技术

Key words:  Shenxue capsules  ginsenoside Rb₁  macroporous adsorptive resin  techology of purification

摘要:

目的 研究大孔吸附树脂富集参血胶囊中人参皂苷Rb₁的工艺条件。方法 采用HP1100高效液相色谱仪ZORBAX SB-C₁₈柱(4.6 mm×250 mm，5 μm)，紫外检测波长：203 nm，流动相：乙腈-水(30∶70)对人参皂苷Rb₁进行测定。以树脂的比吸附量、比洗脱量及人参皂苷Rb₁含量为指标，对树脂类型进行筛选，同时进行洗涤溶剂的选择，及应用L₉(3⁴)正交实验进行洗脱条件的研究，从而确定大孔吸附树脂纯化工艺参数。结果 人参皂苷Rb₁在0.752~9.4 μg内呈现良好的线性关系，确定洗涤溶剂为0.5 mol·L^-1 NaOH溶液，最佳洗脱条件：提取液上柱后静置12 h，用15倍60%的乙醇溶液，以流速1.0 mL·min^-1进行洗脱。在此条件下，所得的人参皂苷Rb₁含量较高。结论 该法简便、专属性强，可用于参血胶囊中人参皂苷Rb₁的提取。

Abstract:

OBJECTIVE To study the technological parameters of the purification process of ginsenoside Rb₁ in Shenxue capsules by macroporous adsorption resin. METHODS Measured the content of ginsenoside Rb₁ by HP1100, the HPLC conditions were ZORBAX SB-C₁₈ column(4.6 mm×250 mm, 5 μm); the detector wavelength was 203 nm; the mobile phase was acetonitrile-water(30∶70). The type of resin was selected by the resin adsorption, elution volume ratio and the content of ginsenoside Rb₁. At the same time, wash solvent was selected and the L₉(3⁴) orthogonal experiment was carried out to study the elution conditions, in order to determine the purification technological parameters of macroporous adsorption resin. RESULTS The linear of ginsenoside Rb₁ had a good relationship from 0.752 μg to 9.4 μg. Determine the washing solvent solution was 0.5 mol·L^-1 NaOH and the optimal elution conditions were: extraction after standing for 12 h in column, with 15 times 60% ethanol, and the fiowrate was 1.0 mL·min^-1. CONCLUSION The method was simple and exclusive, which can be available to enrich ginsenoside Rb₁ in Shenxue capsules.