当归挥发油对小鼠急性心肌缺血损伤的保护作用

Protective Effect of Angelicae Sinensis Radix Volatile Oil on Acute Myocardial Ischemic Injury in Mice

关键词:  当归挥发油  急性心肌缺血  保护作用

Key words:  Angelicae Sinensis Radix volatile oil  acute myocardial ischemia  protective effect

摘要:

目的 研究当归挥发油对垂体后叶素所致小鼠急性心肌缺血损伤的保护作用。方法 昆明种小鼠50只，随机分为对照组、模型组、当归挥发油高、低剂量组和阳性对照组。连续灌胃20 d后，采用垂体后叶素(20 U·kg^-1)腹腔注射建立小鼠急性心肌缺血损伤模型，监测造模前后心电图Ⅱ导联J点变化；生化方法检测血清过氧化氢酶(CAT)、谷胱甘肽转移酶(GSH-Px)、超氧化物歧化酶(SOD)活性，以及乳酸(LD)和丙二醛(MDA)水平；Nagar-Olsen染色观察心肌缺血损伤程度。结果 与模型组比较，各治疗组缺血所致心电图J点的上移均显著降低(P<0.01)；血清CAT、GSH-Px、SOD活力增加(P<0.05)，LD和MDA含量降低(P<0.05)；心肌缺血损伤面积减少。结论 当归挥发油对小鼠急性心肌缺血损伤具有保护作用。

Abstract:

OBJECTIVE To study the protective effect of Angelicae Sinensis Radix volatile oil on pituitrin-induced acute myocardial ischemic injury in mice. METHODS The mice were randanly divided into normal group, model group, high-, low-dose of Angelicae Sinensis Radix volatile oil, and Yinxingye pian group. The acute myocardial ischemic injury model was made by injecting pituitrin in mice, the height of the J spot was measured by electrocardiogram, serum was afforded for biochemical detections, the heart were fixed for pathological investigation to observe the degree of myocardial ischemic injury. RESULTS Compared with the model group, the moving up of J spots in the treated groups was significantly inhibited(P<0.01); the activities of SOD, GSH-Px and CAT increased, while the content of LD and MDA decreased(P<0.05); Nagar-Olsen staining showed that high- and low-dose of Angelicae Sinensis Radix volatile oil significantly diminished the areas of cardiac muscles injured by ischemia. CONCLUSION Angelica volatile oil has protective effect on acute myocardial ischemic injury in mice.