HPLC-ELSD同时测定跌打丸中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量

Simultaneous Determination of Notoginsenoside R1, Ginsenoside Rg1 and Ginsenoside Rb1 in Dieda Pills by HPLC-ELSD

关键词:  跌打丸  三七皂苷R₁  人参皂苷Rg₁  人参皂苷Rb₁  含量测定

Key words:  Dieda pills  notoginsenoside R₁  ginsenoside Rg₁  ginsenoside Rb₁  content determination

摘要:

目的 建立同时测定跌打丸中三七皂苷R₁、人参皂苷Rg₁和人参皂苷Rb1含量的HPLC-ELSD检测方法。方法 采用Phenomenex Kinetex C₁₈色谱柱(100 mm×4.6 mm，2.6 μm)，柱温30 ℃，流速0.5 mL·min^-1，流动相为乙腈-水，梯度洗脱，ELSD检测器，漂移管温度110 ℃，载气(空气)体积流量3.0 L·min^-1。结果 三七皂苷R₁、人参皂苷Rg₁、人参皂苷Rb1分别在0.158~3.16 μg(r=0.999 8)，0.407~8.14 μg(r=0.999 5)，0.446~8.92 μg(r=0.999 9)内呈良好的线性关系，平均加样回收率分别为97.55%(RSD=1.04%)，98.09%(RSD=1.03%)，97.34%(RSD=0.81%)。结论 该方法简便、快速、准确，可用于跌打丸的质量控制。

Abstract:

OBJECTIVE To establish a quantitative method using HPLC-ELSD to simultaneously determine three components(notoginsenoside R₁, ginsenoside Rg₁, ginsenoside Rb₁) in Dieda pills. METHODS A Phenomenex Kinetex C₁₈ column(100 mm×4.6 mm, 2.6 μm) was used to perform the determination, which was maintained at 30 ℃ throughout the analysis. Moble phase was composed of acetonitrile and water with flow rate at 0.5 mL·min^-1 under gradient elution. The evaporation light-scattering detector was used with its drift tube temperature at 110 ℃ and the gas flow rate: 3.0 L·min^-1. RESULTS Notoginsenoside R₁, ginsenoside Rg₁, ginsenoside Rb₁ shown good linearity within 0.158-3.16 μg(r=0.999 8), 0.407-8.14 μg(r=0.999 5), 0.446-8.92 μg(r=0.999 9), and their average recoveries were 97.55%(RSD=1.04%), 98.09%(RSD= 1.03%), 97.34%(RSD=0.81%), respectively. CONCLUSION The method is simple, rapid and accurate. It show this method could be used for the quality control of Dieda Pills.