HPLC同时测定四季三黄片中芦荟大黄素、黄芩苷、黄柏酮和西红花苷-I的含量

Simultaneous Determination of Aloe-emoidin, Baicalin, Obacunone and CrocinⅠin Sijisanhuang Tablets by HPLC

关键词:  四季三黄片  芦荟大黄素  黄芩苷  黄柏酮  西红花苷-Ⅰ  高效液相色谱法

Key words:  Sijisanhuang tablets  aloe-emoidin  baicalin  obacunone  crocinⅠ  HPLC

摘要:

目的 采用HPLC同时测定四季三黄片中芦荟大黄素、黄芩苷、黄柏酮和西红花苷-I的含量。方法 采用welch Topsil-C₁₈色谱柱(4.6 mm×250 mm，4 μm)；流动相：甲醇-0.2%磷酸水溶液；梯度洗脱；检测波长分别为254 nm(芦荟大黄素、黄芩苷、黄柏酮)和440 nm(西红花苷-I)；流速：1.0 mL·min^-1；柱温：30 ℃。结果 芦荟大黄素、黄芩苷、黄柏酮和西红花苷-I线性范围分别为0.079 10~1.582 μg·mL^-1(r=0.999 5)，0.167 5~3.350 μg·mL^-1(r=0.999 8)，0.097 92~1.958 μg·mL^-1(r=0.999 7)和0.033 57~0.671 5 μg·mL^-1(r=0.999 4)，平均加样回收率分别为95.9%(RSD=0.7%)，97.5%(RSD=0.8)，96.4%(RSD=1.0%)，95.5%(RSD=1.3%)。结论 该方法简便、准确、专属性强、重复性好，可用于四季三黄片中芦荟大黄素、黄芩苷、黄柏酮和西红花苷-I的定量分析。

Abstract:

OBJECTIVE To establish an HPLC method for the simultaneous determination of aloe-emoidin, baicalin, obacunone and crocinⅠ in Sijisanhuang tablets. METHODS A Welch Topsil-C₁₈ column(4.6 mm×250 mm, 4 μm) was used with the mobile phase of methanol-0.2% phosphate solution at a flow rate of 1.0 mL·min^-1, the column temperature was 30 ℃ and the detection wavelength was 254 nm for aloe-emoidin, baicalin, obacunone and 440 nm for crocin Ⅰ. RESULTS The calibration curves of aloe-emoidin, baicalin, obacunone and crocin Ⅰ were linear over the ranges of 0.079 10-1.582 μg·mL^-1(r=0.999 5), 0.167 5-3.350 μg·mL^-1(r=0.999 8), 0.097 92-1.958 μg·mL^-1(r=0.999 7), 0.033 57-0.671 5 μg·mL^-1(r=0.999 4), respectively, and the average recoveries were 95.9%(RSD=0.7%), 97.5%(RSD=0.8), 96.4%(RSD=1.0%), 95.5%(RSD=1.3%), respectively. CONCLUSION The method is simple, accurate, specific and repeatable, which can be used for quantitative analysis of aloe-emoidin, baicalin, obacunone and crocinⅠin Sijisanhuang tablets.