HPLC同时测定宣肺止咳合剂3种指标成分的含量

Study on Simultaneous Determination of Three Main Marker Components in Xuanfeizhike Mixture by HPLC

关键词:  宣肺止咳合剂  高效液相色谱法  葛根素  大豆苷  甘草酸  含量测定

Key words:  Xuanfeizhike mixture  HPLC  puerarin  daidzein  glycyrrhizic acid  content determination

摘要:

目的 建立反相高效液相色谱法同时测定宣肺止咳合剂中葛根素、大豆苷、甘草酸的含量。方法 采用Agilent Zorbax SB-C₁₈(4.6 mm×250 mm，5 μm)色谱柱，检测波长：254 nm，以乙腈-0.1%甲酸水为流动相，梯度洗脱，柱温为30 ℃。结果 葛根素、大豆苷、甘草酸浓度分别在99.14~991.4，22.50~225.0，22.60~226.0 μg·mL^-1内与峰面积呈良好的线性关系(r≥0.999 8)，平均回收率分别为99.2%，98.8%，99.3%。结论 该方法简便、准确、重复性好，为宣肺止咳合剂的质量评价与质量控制提供了科学依据。

Abstract:

OBJECTIVE To establish an HPLC method for simultaneous determination of puerarin, daidzein and glycyrrhizic acid in Xuanfeizhike mixture. METHODS Isolation of sample was achieved on an Agilent Zorbax SB-C₁₈(4.6 mm×250 mm, 5 μm), under the 254 nm detection wavelength, gradient eluted by acetonitrile-0.1% formic acid, and the column temperature was maintained at 30 ℃. RESULTS The linearity ranges of puerarin, daidzein and glycyrrhizic acid were 99.14-991.4, 22.50-225.0, 22.60-226.0 μg·mL^-1, respectively(r≥0.999 8). The average recoveries(n=9) of them were 99.2%, 98.9%, 99.3%. CONCLUSION The method is simple, accurate and has a good repeatability. The study provides scientific foundation for quality evaluation and quality control standards for Xuanfeizhike mixture.