响应面法优化斑花黄堇中总生物碱的提取工艺

Optimization of Ultrasonic Extraction of Total Alkaloids from Corydalis conspersa Maxim

关键词:  斑花黄堇  生物碱  提取工艺  响应面法

Key words:  Corydalis conspersa Maxim  alkaloid  extraction process  response surface methodology

摘要:

目的 优化盐酸回流提取藏药斑花黄堇中总生物碱的提取工艺。方法 根据单因素试验结果，选择影响总生物碱提取效果较大的盐酸浓度、液料比、提取温度为自变量，以总生物碱的得率为响应值，进行Box-Benhken中心组合试验设计，采用响应面法评估这些因素对总生物碱提取效果的影响。结果 提取斑花黄堇中总生物碱的最佳工艺条件为液料比35 mL·g^-1，盐酸浓度为0.4%，提取温度为75℃，得斑花黄堇中总生物碱的提取率为0.46 mg·g^-1。结论 该法优化的提取工艺科学可靠，可用于斑花黄堇总生物碱的提取生产。

Abstract:

OBJECTIVE To optimize ultrasonic-assisted extraction process of total alkaloids from Corydalis conspersa Maxim. METHODS the hydrochloric acid concentration, liquid-feed ratio and extraction temperature, which were major factors influencing the extraction according to the single factor experiments, were selected as independent variables, and through using the extraction rate of total alkaloids as response values and making the Box-Benhken central combinational design, the effects of the above factors on the extraction were estimated by response surface methodology. RESULTS The results indicated that the optimal extraction conditions were as follows:35 mL·g^-1 of liquid-feed ratio, 0.4% of hydrochloric acid concentration, and 75℃ extraction temperature. Under these conditions, the content of total alkaloids was 0.46 mg·g^-1 from Corydalis conspersa Maxim by ultraviolet spectrophotometer. CONCLUSION The optimized parameters from the expriments are accurate, which can be used for the extraction and production of total alkaloids from Corydalis conspersa Maxim.