白术醇提物的抗炎镇痛活性研究

Study on Anti-inflammatory and Analgesic Effect of Ethanol Extract of Atractylodes Macrocephala Koidz

关键词:  白术  醇提物  抗炎  镇痛

Key words:  Atractylodes macrocephala Koidz.  ethanol extract  anti-inflammatory  analgesic

摘要:

目的 探讨白术醇提物的抗炎镇痛活性。方法 采用热板法测定白术不同剂量组小鼠的痛阈值，腹腔注射0.6%醋酸刺激致痛模型（扭体法）观察白术3个不同剂量的镇痛作用；采用二甲苯致小鼠耳廓肿胀实验和角叉菜胶致大鼠足肿胀实验观察3个不同剂量白术的抗炎作用。结果 高、中剂量的白术醇提物可显著增加小鼠的热板痛阈值（P<0.01），减少腹腔注射醋酸引起的小鼠扭体反应次数（P<0.01），而白术低剂量组不能有效的提高小鼠的痛阈值和减少扭体反应次数。在抗炎试验中，高、中剂量的白术醇提物可显著抑制小鼠耳廓肿胀度（P<0.01），而低剂量组对小鼠耳廓肿胀抑制效果不明显；高剂量组在2 h后能显著抑制大鼠足跖肿胀，中剂量组（除6 h时间点）与白术醇提物低剂量组（除3 h时间点）在药后0.5~6 h之间与模型组比较均无显著性差异。结论 白术醇提物具有较好的抗炎、镇痛作用，并呈现一定的剂量依赖性。

Abstract:

OBJECTIVE To investigate the analgesic and anti-inflammatory activities of the ethanol extract of Atractylodes macrocephala Koidz. (EAM). METHODS Analgesic activity of EAM was evaluated by hot-plate test and acetic acid-induced writhing test on ICR mice, while anti-inflammatory activity of EAM was assessed by xylene-induced ear edema on ICR mice and carrageenan-induced rat paw edema on SD rats. RESULTS High and medium doses of the EAM could prolong the threshold time of pain(P<0.01), reduce the writhing times induced by acetic acid(P<0.01), and inhibit the edema induced by xylene and canageenan(P<0.01), while low dose of the EAM showed no siginificance differences compared to the control group in all experiments. In carrageenan-induced paw edema model, high dose of EAM could inhibit the paw swelling significantly since 2 h of administration, and in the period of 0.5-6 h after drug administration, medium dose of EAM (with the exception of 6 h) and low dose of EAM (with the exception of 3 h) had no significant differences compared with model group. CONCLUSION The ethanol extract of EAM has analgesic and anti-inflammatory effects, and present the dose-response relationship.