猴头菌-地龙生物转化物体内外抗凝血活性研究

Study on Anticoagulant Activity of Hericium Erinaceus-Pheretima Biotransformation in Vivo and in Vitro

关键词:  猴头菌-地龙生物转化物  猴头菌  地龙  逐瘀通脉胶囊  抗凝血活性

Key words:  Hericium erinaceus-Pheretima biotransformation  Hericium erinaceus  Pheretima  Zhuiyu Tongmai capsule  anticoagulant ativity

摘要:

目的 探讨猴头菌-地龙（HD）生物转化物体内外抗凝血活性。方法 以华法林钠为西药阳性对照组，以逐瘀通脉胶囊为中药阳性对照组，以地龙和猴头菌为HD生物转化物的原料药材对照组，生理盐水为阴性对照组，将36只健康家兔分6组分别灌胃各组药物水煎液或混悬液，30~40 min后耳缘静脉采血，离心，将血浆置于凝血分析仪上测定凝血活酶时间和凝血酶原时间，进行统计分析。结果 在给定剂量下，体内抗凝血活性：华法林钠片 > HD生物转化物 > 地龙≈逐瘀通脉胶囊 > 生理盐水≈猴头菌，体外抗凝血活性：HD生物转化物 > 地龙 > 猴头菌≈华法林钠片≈生理盐水≈逐瘀通脉胶囊。结论 HD生物转化物有显著的抗凝血活性，有良好的开发应用前景。

Abstract:

OBJECTIVE To study the anticoagulant activity of Hericium Erinaceus-Pheretima(HD) biotransformation in vitro and in vivo. METHODS With the warfarin sodium tablets as western medicine contrast group, the Zhuyu Tongmai capsule as traditional Chinese medicine contrast group, the Pheretima and Hericium erinaceus as raw material contrast group, normal saline as a negative contrast group, and divide 36 healthy rabbits into 6 groups with gavaging decoction or suspension, then take the blood from marginal ear vein after 30-40 min and centrifuge it, and test the prothrombin time, and activated partial thromboplastin time, value of the centrifugal serum. Finally, do the statistical analysis. RESULTS In vivo anticoagulation activity:warfarin sodium tablets > HD biotransformation > Pheretima≈Zhuiyu Tongmai capsule > normal saline≈Hericium erinaceus. In vitro anticoagulation activity:HD biotransformation > Pheretima > Hericium erinaceus≈warfarin sodium tablets≈normal saline≈Zhuiyu Tongmai capsule. CONCLUSION HD biotransformation have a significant anticoagulant activity, and have a good development prospect.