补肾活血方对兔胫骨截骨延长骨组织形态及骨密度的影响

Effect of Bushen Huoxue Decoction on Bone Tissue Morphology and Bone Mineral Density in Rabbit Tibia Lengthened

关键词:  补肾活血方  截骨延长  组织形态  骨密度  TGF-β2

Key words:  Bushen Huoxue decoction  limb lengthen  tissue morphology  bone mineral density  TGF-β2

摘要:

目的 探索补肾活血方对兔胫骨截骨延长术后延长区域骨组织形态及骨密度的作用及机制。方法 24只新西兰大白兔完全随机方法分为假手术组、模型组和补肾活血方组，每组8只大白兔，假手术组和模型组均予以生理盐水灌胃，补肾活血组予以补肾活血方灌胃。干预8周后处死，观察各组兔骨延长区域骨组织形态、骨密度、骨组织形态计量学及TGF-β2基因表达水平。结果 补肾活血方组延长区骨组织形态相比模型组，胫骨外侧的骨皮质结构连续完整，内外层骨皮质间的骨质明显增多、增厚，骨小梁较粗且排列整齐、均匀，破裂程度较低，血管数量增多，网状新骨生成明显。假手术组兔胫骨延长区骨密度高于模型组和补肾活血方组（P<0.05），补肾活血方组兔胫骨延长区骨密度要高于模型组，差异具有统计学意义（P<0.05）；假手术组兔胫骨延长区骨小梁面积百分数、骨小梁数量和骨小梁厚度均要大于模型组和补肾活血方组，骨小梁分离度小于模型组和补肾活血方组，差异具有统计学意义（P<0.05）；补肾活血方组兔胫骨延长区骨小梁面积百分数、骨小梁数量和骨小梁厚度骨要大于模型组，骨小梁分离度小于模型组，差异具有统计学意义（P<0.05）；模型组和补肾活血方组TGF-β2基因表达水平均要高于假手术组，差异具有统计学意义（P<0.05）；而模型组TGF-β2基因表达水平低于补肾活血方组，差异具有统计学意义（P<0.05）。结论 补肾活血方可能通过刺激TGF-β2的表达，增加骨延长区的成骨作用，促进骨的矿化，以保证截骨延长区的顺利愈合。

Abstract:

OBJECTIVE To explore the effect and mechanism of Bushen Huoxue decoction on bone tissue morphology and bone mineral density (BMD) in rabbit tibia lengthened. METHODS Twenty-four New Zealand white rabbits were randomly divided into sham operation group, model group and Bushen Huoxue group, 8 rabbits in each group. The sham operation group and model group were given physiological saline gavage, and the Bushen Huoxue group were given Bushen Huoxue decoction gavage. After eight weeks intervention, all rabbits were killed, and tibial lengthen regional bone tissue morphology, BMD, bone histomorphometry and gene expression of TGF-β2 were observed. RESULTS Compared with model group, there was continuous and intact tibia bone cortex in the Bushen Huoxue group, and bone substance of both inner and outer layer bone cortex increased significantly. Its bone trabecular was more thicken, regular arrangement, uniform distribution, lower rupture degree, more blood vessels and obvious reticular bone formation. BMD of bone lengthen area in sham operation group was higher than those of model group and Bushen Huoxue group(P<0.05), but BMD of bone lengthen area in Bushen Huoxue group was higer than that of model group (P<0.05). Tb.Ar, Tb.N, Tb.Th of bone lengthen area in sham operation group were greater than those of model group and Bushen Huoxue group, and Tb.Sp was less than that of model group and Bushen Huoxue group (P<0.05). But Tb.Ar, Tb.N, Tb.Th of bone lengthen area in Bushen Huoxue group were greater than that of model group, and Tb.Sp was less than that of model group(P<0.05). Gene expression of TGF-β2 in model group and Bushen Huoxue group were greater than that of sham operation group(P<0.05), but gene expression of TGF-β2 in model group was less than that of Bushen Huoxue group(P<0.05). CONCLUSION Bushen Huoxue decoction may play osteogenic action and promote bone mineralization through stimulating TGF-β2 expression, which is in order to ensure osteotomy lengthening region healing.