清凉膏促进肛肠术后感染切口愈合的机制研究

Study on the Mechanism of Qingliang Ointment Promoting Healing of Anorectal Postoperative Infection

关键词:  清凉膏  肛肠术后感染切口  血管内皮生长因子  血管紧张素-2  CD34  羟脯氨酸

Key words:  Qingliang ointment  anorectal postoperative infection incision  vascular endothelial growth factor (VEGF)  angiopoientin-2(ANG-2)  CD34  hydroxyproline

摘要:

目的 观察清凉膏对肛肠手术后感染切口肉芽组织中血管内皮生长因子（vascular endothelial growth factor，VEGF）、血管紧张素-2（angiopoientin-2，ANG-2）、CD34的表达和羟脯氨酸含量的影响。方法 42例肛肠手术患者随机分为对照组和清凉膏组，对照组患者术后创面用凡士林纱条外敷，清凉膏组用清凉膏纱条外敷，术后第3，7，14天取创面肉芽组织检测VEGF、ANG-2、CD34的表达和羟脯氨酸含量。结果 术后治疗第7，14天的肉芽组织与治疗第3天比较，对照组和清凉膏组中VEGF、ANG-2、CD34蛋白的表达和羟脯氨酸含量逐渐增多（P<0.05）；术后治疗第3，7天，清凉膏组与对照组在创面肉芽组织中VEGF、ANG-2、CD34蛋白表达和羟脯氨酸含量无明显差异；术后第14天，清凉膏组与对照组比较，创面肉芽组织中VEGF、ANG-2、CD34蛋白表达和羟脯氨酸含量明显增多（P<0.05）。结论 清凉膏可能通过促进VEGF、ANG-2、CD34蛋白的表达和促羟脯氨酸含量增加发挥促进创面愈合的作用。

Abstract:

OBJECTIVE To observe the effect of Qingliang ointment on the expression of vascular endothelial growth factor (VEGF),angiopoientin-2(ANG-2),CD34 and hydroxyproline content in granulation tissue of incisional wound after anorectal operation.METHODS Fourty-two cases of anorectal surgery patients were randomly divided into control group and Qingliang ointment group,the control group of patients with external application of Vaseline gauze wound,while the Qingliang ointment group with external application of Qingliang ointment.The expression of VEGF,ANG-2,CD34 and hydroxyproline content were detected on the 3rd day,7th day and 14th day after operation.RESULTS Compared with the 3rd day,the expression of VEGF,ANG-2 and CD34 and the content of hydroxyproline in control group and Qingliang ointment group were gradually increased (P<0.05) on the 7th day and the 14th day after operation in the granulation tissue.The expression of VEGF,ANG-2,CD34 protein and hydroxyproline in the wound granulation tissue of Qinglian ointment group and control group had no significant difference on the 3rd and 7th day after operation.The expression of VEGF,ANG-2,CD34 and the content of hydroxyproline in granulation tissues of wound group were increased (P<0.05) on the 14th day after operation compared with the control group.CONCLUSION Qingliang ointment can promote the wound healing by promoting the expression of VEGF,ANG-2,CD34 protein and the increase of hydroxyproline content.