齐墩果酸通过抑制NF-κB/ICAM-1信号通路减轻大鼠蛛网膜下腔出血后早期脑损伤的研究

Effects of Oleanolic Acid on Early Brain Injury Following Subarachnoid Hemorrhage in Rats by Inhibiting NF-κB/ICAM-1 Signaling Pathway

关键词:  蛛网膜下腔出血  齐墩果酸  早期脑损伤  NF-κB/ICAM-1信号通路

Key words:  subarachnoid hemorrhage  oleanolic acid  early brain injury  NF-Kb/ICAM-1 signaling pathway

摘要:

目的 研究齐墩果酸（oleanolic acid，OA）对蛛网膜下腔出血（subarachnoid hemorrhage，SAH）后早期脑损伤的影响，并探讨其潜在的作用机制。方法 39只大鼠随机分为假手术组、模型组、OA治疗组（OA组，20 mg·kg^-1），每组13只。分别对各组进行体质量测量，神经功能评分，伊文思蓝法检测血脑屏障的通透性，并用Western blot法检测大鼠脑皮质中NF-κB/ICAM-1信号通路蛋白的表达情况。结果 与模型组比较，OA组能显著增加大鼠体质量和神经功能评分，显著降低脑部伊文思蓝通透率和NF-κB/ICAM-1信号通路蛋白的表达水平，差异均具有统计学意义（P<0.01或P<0.05）。结论 齐墩果酸能够明显减轻大鼠SAH后早期脑损伤，有可能是通过减少Akt/NF-κB/ICAM-1信号通路的炎症因子发挥作用。

Abstract:

OBJECTIVE To study the effects of oleanolic acid (OA) on brain damage after subarachnoid hemorrhage (SAH) and to explore its potential mechanism. METHODS Thirty-nine rats were randomly divided into sham group, model group and oleanolic acid treatment group (OA group, 20 mg·kg^-1), each group of 13. Weight measurement, neurological function score, and Evans blue method to detect the permeability of blood-brain barrier were used for each group, separately. Then the protein expression of NF-κB, p65 and ICAM-1 in the cerebral cortex of rats were detected by Western blot. RESULTS Compared with the model group, the body weight and neurological score were improved, the Evans blue rate and the level of NF-κB/ICAM-1 signaling pathway were reduced in the OA group, significantly(P<0.01 or P<0.05). CONCLUSION OA could attenuate early brain injury in SAH rats by decreasing the inflammatory cytokines of Akt/NF-κB/ICAM-1 signaling pathway.