不同配比白芍、桂枝多组分大鼠肠渗透特性及其相互影响

Intestinal Penetration Characteristics and Mutual Effects of Multi-component of Cinnamomi Ramulus and Paeoniae Radix Alba with Different Ratio in Rats

关键词:  桂枝-白芍药对  外翻肠囊法  渗透特性  指纹图谱  HPLC

Key words:  Cinnamomi Ramulus-Paeoniae Radix Alba pair  everted gut sac method  permeability  fingerprint  HPLC

摘要:

目的 考察白芍、桂枝及其不同配伍比例水煎液中的成分在大鼠体外的肠吸收特性及其可能存在的相互作用。方法 采用大鼠外翻肠囊法结合HPLC指纹图谱定性、定量考察肠渗透前后各化学成分的变化以及白芍桂枝不同配比的相互影响。结果 桂枝各成分大多吸收良好（P_app>1×10^-5 cm·s^-1），组分间渗透前后的相对比例基本未变；白芍中以芍药苷为代表的亲水性成分吸收较好（P_app>1×10^-6 cm·s^-1），但成分随保留时间延长渗透变差；配伍前后二者各成分的渗透未发生明显的变化。结论 除白芍部分亲脂性成分外，桂枝与白芍中大多成分肠道吸收较好，二者配伍对各成分的吸收影响不大。

Abstract:

OBJECTIVE To investigate the in vitro intestinal absorption characteristics of the components in the decoction of Cinnamomi Ramulus and Paeoniae Radix Alba with different proportions in rats and the possible interaction of the components. METHODS The in vitro everted gut sac model combined with HPLC fingerprint were used to determine the change of chemical composition qualitatively and quantitatively before and after intestinal invasion as well as mutual influence of the different ratio of Cinnamomi Ramulus and Paeoniae Radix Alba. RESULTS The results showed that the most components of Cinnamomi Ramulus had good intestinal absorption (P_app>1×10^-5 cm·s^-1), and the ratio of the components was not changed significantly before and after infiltration. The absorption of hydrophilic components represented by paeoniflorin in Paeoniae Radix Alba was better(P_app>1×10^-6 cm·s^-1), but the permeability was deteriorated with the increase of the retention time. The permeation of the two components did not change obviously before and after infiltration. CONCLUSION In addition to some lipophilic components of Paeoniae Radix Alba, most of the components of Cinnamomi Ramulus and Paeoniae Radix Alba are absorbed well, and the combination of the two has little effect on the absorption of each component.