HPLC同时测定不同产地番石榴叶药材中4种黄酮类成分含量

Simultaneous Determination of 4 Flavonoids in Psidium Guajava Leaves from Different Habitats by HPLC

关键词:  番石榴叶  黄酮  高效液相色谱法

Key words:  Psidium guajava leaves  flavonoids  HPLC

摘要:

目的 建立同时测定不同产地番石榴叶药材中金丝桃苷、槲皮素-3-O-β-D-吡喃阿拉伯糖苷、槲皮素-3-O-α-L-呋喃阿拉伯糖苷、槲皮素含量的方法。方法 采用色谱柱为Agilent Eclipse XDB-C₁₈（4.6 mm×250 mm，5 μm），流动相为甲醇-0.2%磷酸水（梯度洗脱），流速为1 mL·min^-1，柱温为35℃，检测波长为360 nm，进样量10µ；L。结果 金丝桃苷、槲皮素-3-O-β-D-吡喃阿拉伯糖苷、槲皮素-3-O-α-L-呋喃阿拉伯糖苷、槲皮素检测进样量线性范围分别为0.155~3.100 μg （r=1.000 0），0.159~3.182 μg （r=0.999 8），0.180~3.601 μg （r=0.999 8），0.007~0.135 μg （r=1.000 0）；精密度，稳定性，重复性试验的RSD<2.0%；加样回收率分别为100.32%（RSD=1.44%，n=6），97.79%（RSD=1.11%，n=6），100.73%（RSD=1.43%，n=6），98.33%（RSD=1.48%，n=6），10批不同产地的番石榴叶药材中4种黄酮类成分含量差异较大。结论 该方法简便、专属性强、准确度高、重复性好，可用于不同产地番石榴叶药材的质量控制。

Abstract:

OBJECTIVE To establish a method for the simultaneous determination of 4 flavonoids in Psidium guajava leaves. METHODS HPLC was performed on the column of Eclipse XDB-C₁₈ with mobile phase of methanol-0.2% phosphate(gradient elution) at a flow rate of 1.0 mL·min^-1, the detection wavelength was 360 nm, column temperature was 35℃ and injection volume was 10 μL. RESULTS The linear range was 0.155-3.100 μg for hyperin(r=1.000 0), 0.159-3.182 μg for quercetin-3-O-β-D-arabinopyranoside (r=0.999 8), 0.180-3.601 μg for quercetin-3-O-α-L-furanarabino side ethyl acetate (r=0.999 8),0.007-0.135 μg for quercetin (r=1.000 0); RSDs of precision, stability and reproducibility were lower than 2.0%; recoveries were 100.32%(RSD=1.44%, n=6), 97.79%(RSD=1.11%, n=6), 100.73%(RSD=1.43%, n=6), 98.33%(RSD=1.48%, n=6), respectively. There were quite large differences in the contents of 4 flavonoids in Psidium guajava leaves from different habitats. CONCLUSION The method is simple with good specific, precision and repeatability, can be used for the quality control of Psidium guajava leaves from different habitats.