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引用本文:黄文康,岳超,宋剑锋,丁国琴,张文婷,赵维良.HPLC同时测定衢枳壳中7种指标成分的含量[J].中国现代应用药学,2018,35(3):404-407.
HUANG Wenkang,YUE Chao,SONG Jianfeng,DING Guoqin,ZHANG Wenting,ZHAO Weiliang.Simultaneous Determination of Seven Constituents in Citrus changshanhuyou Y. B. Chang by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(3):404-407.
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HPLC同时测定衢枳壳中7种指标成分的含量
黄文康1, 岳超2, 宋剑锋3, 丁国琴4, 张文婷2, 赵维良2
1.浙江中医药大学, 杭州 310053;2.浙江省食品药品检验研究院, 杭州 310052;3.衢州市食品药品检验研究院, 浙江 衢州 324000;4.杭州市中医院, 杭州 310007
摘要:
目的 建立HPLC同时测定衢枳壳中芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、木犀草素、川陈皮素和桔皮素含量。方法 采用Agilent Extend C18(4.6 mm×250 mm,5 μm)色谱柱,检测波长:330 nm,以乙腈-0.1%甲酸溶液为流动相,梯度洗脱,流速:1 ml·min-1,柱温:30℃。结果 芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、木犀草素、川陈皮素、桔皮素分别在23.30~1 164.80 ng,170.84~8 541.91 ng,17.22~861.20 ng,156.17~7 808.64 ng,3.13~156.48 ng,0.90~45.23 ng,0.85~42.27 ng内线性关系良好(r ≥ 0.999 5,n=6),平均回收率分别为98.8%,101.3%,98.3%,96.8%,101.8%,101.7%,108.9%。结论 首次建立了衢枳壳中7个成分HPLC含量测定方法,该方法简便,结果准确,可为综合评价衢枳壳质量提供依据。
关键词:  衢枳壳  高效液相色谱法  芸香柚皮苷  柚皮苷  橙皮苷  新橙皮苷  木犀草素  川陈皮素  桔皮素
DOI:10.13748/j.cnki.issn1007-7693.2018.03.021
分类号:R917.101
基金项目:
Simultaneous Determination of Seven Constituents in Citrus changshanhuyou Y. B. Chang by HPLC
HUANG Wenkang1, YUE Chao2, SONG Jianfeng3, DING Guoqin4, ZHANG Wenting2, ZHAO Weiliang2
1.Zhejiang Chinese Medical University, Hangzhou 310053, China;2.Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China;3.Quzhou Institute for Food and Drug Control, Quzhou 324000, China;4.Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou 310007, China
Abstract:
OBJECTIVE To establish a method for the simultaneous determination of the contents of narirutin, naringin, hesperidin, neohesperidin, luteolin, nobiletin and hesperetin of Citrus changshan-huyou Y.B.Chang by HPLC. METHODS The HPLC method was adopted with Agilent Extend C18 column (250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile and 0.1% methanoic acid with gradient elution. The flow rate was 1 mL·min-1. The detection wavelength was 330 nm and column temperature was 30℃. RESULTS The linear relation of narirutin, naringin, hesperidin, neohesperidin, luteolin, nobiletin and hesperetin was excellent within the range of 23.30-1 164.80 ng, 170.84-8 541.91 ng, 17.22-861.20 ng, 156.17-7 808.64 ng, 3.13-156.48 ng, 0.90-45.23 ng, 0.85-42.27 ng (r ≥ 0.999 5, n=6), and the mean recovery was 98.8%, 101.3%, 98.3%, 96.8%, 101.8%, 101.7%, 108.9%. CONCLUSION It is the first time to establish the HPLC method of the simultaneous determination for the seven contents of Citrus changshan-huyou Y.B.Chang. The method is simple, reliable and accurate, which provides the reference for the quality control for Citrus changshan-huyou Y.B.Chang.
Key words:  Citrus changshan-huyou Y.B.Chang  HPLC  narirutin  naringin  hesperidin  neohesperidin  luteolin  nobiletin  hesperetin
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