| 引用本文: | 王银环,钱凌,董芳华,李珏,陈敏,郑小玲.流式细胞术和平板计数法用于地衣芽孢杆菌活菌制剂检测的比较研究[J].中国现代应用药学,2018,35(3):352-356. |
| WANG Yinhuan,QIAN Ling,DONG Fanghua,LI Jue,CHEN Min,ZHENG Xiaoling.Comparative Research on the Detection of Viable Organism Preparation of Bacillus licheniformis between Flow Cytometry and Plate Counting Method[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(3):352-356. |
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| 流式细胞术和平板计数法用于地衣芽孢杆菌活菌制剂检测的比较研究 |
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王银环1, 钱凌1, 董芳华2, 李珏1, 陈敏3, 郑小玲1
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1.浙江省食品药品检验研究院, 杭州 310052;2.绍兴市市场监督管理局滨海新城分局, 浙江 绍兴 312000;3.浙江省药品认证检查中心, 杭州 310052
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| 摘要: |
| 目的 考察流式细胞技术和平板计数法2种方法检测地衣芽孢杆菌活菌制剂（片剂、颗粒剂和胶囊剂）中芽孢数的不同。方法 分别采用BD FACSMicroCount全自动微生物分析系统和平板计数法检测不同水浴温度及不同水浴时间处理过的地衣芽孢杆菌活菌胶囊中的芽孢数。同时采用上述2种方法检测3种地衣芽孢杆菌活菌制剂（片剂、颗粒剂和胶囊剂）中的活菌数、芽孢数和芽孢率。结果 流式细胞技术和平板计数法对于检测不同水浴温度及不同水浴时间处理过的地衣芽孢杆菌活菌胶囊中的芽孢数无显著性差异。采用上述2种方法检测的3种地衣芽孢杆菌活菌制剂（片剂、颗粒剂和胶囊剂）中的活菌数、芽孢数和芽孢率的结果比较均无显著性差异。结论 流式细胞技术和平板计数法对于3种地衣芽孢杆菌活菌制剂（片剂、颗粒剂和胶囊剂）检测活菌数、芽孢数和芽孢率无显著性差异。 |
| 关键词: 地衣芽孢杆菌活菌制剂 流式细胞技术 平板计数法 芽孢数测定 活菌数测定 芽孢率 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.03.010 |
| 分类号:R927.12 |
| 基金项目:浙江省药品接触材料质量控制研究重点实验室（2014E10006） |
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| Comparative Research on the Detection of Viable Organism Preparation of Bacillus licheniformis between Flow Cytometry and Plate Counting Method |
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WANG Yinhuan1, QIAN Ling1, DONG Fanghua2, LI Jue1, CHEN Min3, ZHENG Xiaoling1
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1.Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China;2.Market Supervision Administration of Shaoxing Municipality Binhai Branch, Shaoxing 312000, China;3.Zhejiang Center for Drug Inspection, Hangzhou 310052, China
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| Abstract: |
| OBJECTIVE To investigate the differences between flow cytometry(FCM) and plate counting method on detecting the spore count in viable organism preparation of Bacillus licheniformis (tablet, granule and capsule). METHODS BD FACSMicroCount analytical system and plate counting method respectively were used to survey and evaluate the spore count in viable organism capsule of Bacillus licheniformis, which were treated by different water bath temperature or by different water bath time. The methods of above-mentioned were applied to detect the spore count in three viable organism preparation of Bacillus licheniformis (tablet, granule and capsule), at the same time. RESULTS There were no significant differences between FCM and plate counting method, with regard to detect the spore count in viable organism capsule of Bacillus licheniformis which were treated by different water bath temperature or by different water bath time. And there were no significant differences in viable count, spore count and sporulation efficiency of three viable organism preparation of Bacillus licheniformis (tablet, granule and capsule), which were detected by above-mentioned methods. CONCLUSION There are no significant differences between FCM and plate counting method, with regard to detect the viable count, spore count and sporulation efficiency of three viable organism preparation of Bacillus licheniformis (tablet, granule and capsule). |
| Key words: viable organism preparation of Bacillus licheniformis flow cytometry plate counting method spore counting viable counting sporulation efficiency |
