场放大进样-高效毛细管电泳测定中药附子指纹图谱研究

Study on the Fingerprint of Aconitum Carmichaelii Based on FASS-HPCE Technology

关键词:  附子  指纹图谱  高效毛细管电泳

Key words:  Aconitum carmichaelii  fingerprint  high performance capillary electrophoresis

摘要:

目的 建立适用于附子药材饮片的指纹图谱法。方法 采用FASS-HPCE技术对检测条件进行优化，确立高效毛细管电泳色谱分析中药附子质量的指纹图谱方法。采用所建立的中药高效毛细管电泳指纹图谱对10个批次的附子饮片进行考察。结果 经考察确定适于附子指纹图谱研究的电泳条件为背景缓冲液100 mmol·L^-1硼砂-硼酸缓冲溶液（pH 9.0）：甲醇=7：3的电解质溶液；电压进样：20 kV，20 s；分离电压：10 kV；检测波长：230 nm；柱温：25℃。所采集的10批药材中有8个共有峰，其中的3个峰分别指认为苯甲酰次乌头原碱、苯甲酰新乌头原碱、苯甲酰乌头原碱，是附子主要的药效物质。结论 所建立的基于高效毛细管电泳技术的指纹图谱法作为一种快速、有效的分析方法可用于中药附子的研究。

Abstract:

OBJECTIVE To evaluate the quality of Aconitum carmichaelii based on FASS-HPCE technology. METHODS FASS-HPCE technology was used for setting up the fingerprint of Aconitum carmichaelii. And ten samples were analyzed. RESULTS Electrophoretic buffer solution were as follows, 100 mmol·L^-1 borate saline buffer (pH 9.0):menthol=7:3; injection voltage and separation voltage were 20 kV (20 s) and 10 kV, respectively; detection wavelength was 230 nm; and the column temperature was 25℃. The fingerprint yield 8 common peaks, and 3 of them were identified as benzoylhypaconine, benzoylaconine, benzoylmesaconine. They were the main pharmacophore in Aconitum carmichaelii. CONCLUSION FASS-HPCE can be used as a rapid, effective fingerprint chromatography management for the the quality control of Aconitum carmichaelii.