畲药降脂轻身茶的HPLC特征图谱及4个成分含量测定

Study on HPLC Characteristic Spectrum of Jiangzhi Qingshen Tea and Determination of Four Active Components

关键词:  降脂轻身茶  特征图谱  异槲皮苷  山奈酚-3-O芸香糖苷  芦丁  绿原酸  质量控制

Key words:  Jiangzhi Qingshen tea  characteristic spectrum  isoquercitrin  kaempferol-3-O-rutinoside  rutin  chlorogenic acid  quality control

摘要:

目的 采用HPLC建立畲药降脂轻身茶的特征图谱，并同时测定4种成分的含量。方法 以绿原酸、芦丁、异槲皮苷和山奈酚-3-O-芸香糖苷为对照品；采用Agilent ZORBAX SB-C₁₈色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈-0.2%磷酸溶液，线性梯度洗脱，流速为0.8 mL·min^-1，检测波长为360 nm，柱温为40℃。采用中药色谱指纹图谱相似度评价系统对结果进行分析。结果 绿原酸、芦丁、异槲皮苷、山奈酚-3-O芸香糖苷分别在0.072 6~0.726，0.076 3~0.763，0.071~0.71，0.113 1~1.131 μg内呈良好的线性关系，相关系数均≥ 0.999 6；平均加样回收率分别为96.15%，95.62%，95.28%，95.76%。结论 所建立的特征图谱相关性强，可结合4种成分含量测定全面控制畲药降脂轻身茶的质量。

Abstract:

OBJECTIVE To establish an HPLC characteristic spectrum of Jiangzhi Qingshen tea and simultaneous determination of four active components. METHODS With chlorogenic acid, rutin, isoquercitrin and kaempferol-3-O -rutinoside as reference substance, the HPLC analysis was carried on an Agilent Zorbax SB-C₁₈ column (4.6 mm×250 mm, 5 μm) with the column temperature of 40℃; the mobile phase was acetonitrile-0.2% phosphoric acid solution (gradient elution) at the flow rate of 0.8 mL·min^-1; the detection wavelength was 360 nm. The similarity evaluation system for chromatographic fingerprint of TCM was used to calculate the similar degree. RESULTS The linear ranges of chlorogenic acid, rutin, isoquercitrin, and kaempferol-3-O-rutinoside were 0.072 6-0.726, 0.076 3-0.763, 0.071-0.71, 0.113 1-1.131 μg, respectively (r ≥ 0.999 6), and the average recoveries of three components were 96.15%, 95.62%, 95.28%, 95.76%, respectively. CONCLUSION With a good correlation, the characteristic spectrum can be combined with the assay of 4 components to control the quality of Jiangzhi Qingshen tea comprehensively.