HPLC测定不同采收年限雷公藤根及不同部位雷公藤甲素含量

Determination of Triptolide in Different Ages of Root and Different Parts of Tripterygium wilfordii Hook f. by HPLC

关键词:  雷公藤  雷公藤甲素  采收年限  高效液相色谱法  含量测定

Key words:  Tripterygium wilfordii Hook f.  triptolide  harvesting years  HPLC  determination

摘要:

目的 用高效液相色谱法测定雷公藤中雷公藤甲素的含量，研究雷公藤植株不同部位及不同采收年限雷公藤根中雷公藤甲素含量的差异情况。方法 采用外标法，以甲醇-水(45∶55)为流动相；检测波长为218 nm；柱温为28 ℃；流速为1.0 mL·min-1。结果 雷公藤甲素浓度在1.026～10.26 μg·mL-1呈良好的线性关系(r=0.999 9)，回收率为95.90%，RSD=3.49%(n=6)。结论 本法简便、准确、灵敏、重复性良好，适用于雷公藤甲素的含量测定。在不同采收年限中，3年生雷公藤全根中雷公藤甲素含量最高，1年生最低；在不同部位中，根皮中含量最高，其次是叶片，根木质部最低。

Abstract:

OBJECTIVE To determine the content of triptolide in Tripterygium wilfordii Hook f. by HPLC, and to study the content of triptolide in different harvesting years and different parts of Tripterygium wilfordii. METHODS An external standard method was used with the mobile phase of methanol-water (45∶55). The wavelength for the detection was 218 nm and the flow rate was 1.0 mL·min-1. The column temperature was 25 ℃. RESULTS The linear range for the determination of triptolide was 1.026-10.26 μg·mL-1(r=0.999 9). The average recovery was 95.90% and RSD was 3.49% (n=6). CONCLUSION The method is simple and accurate. The results are sensitive and reproducible and may be used in the determination of triptolide in Tripterygium wilfordii Hook f.. The triptolide content is the highest in three-year root and lowest in one-year root. The triptolide content in root cortex is the highest, secondly is in the leaf, and in radical centre is the lowest.