芳竭丸活血化瘀作用的实验研究

Experimental Study on Huoxuehuayu Effects of Fangjiewan

关键词:  芳竭丸  活血化瘀  药理作用

Key words:  Fangjiewan  Huoxuehuayu  pharmacological effect

摘要:

目的对中药新药芳竭丸的活血化瘀作用进行研究。方法采用在冰水中游泳和sc注射肾上腺素方法制造大鼠急性血瘀模型，观察芳竭丸对血液流变性的影响；用肾上腺素制造大鼠肠系膜急性微循环障碍模型，观察芳竭丸对微循环障碍的改善作用；采用体外法，用PT和KPTT试剂盒测定血液凝固时间，观察芳竭丸对凝血酶原时间(PT)及活化部分凝血活酶时间(KPTT)的影响。结果芳竭丸能明显降低大鼠的全血黏度、血浆黏度、血纤维蛋白原含量、血沉及血小板黏附率，减轻血瘀大鼠血液的黏、凝状态，防止血栓形成；可抑制肾上腺素引起的大鼠肠系膜微循环细动脉管径缩小、流速减慢、毛细血管开放量减少、流态改变，并改善这些现象；可显著延长家兔的PT、KPTT。结论芳竭丸有改善血液流变性及微循环障碍的作用，有抑制内源性及外源性凝血系统的作用，提示有活血化瘀作用。

Abstract:

OBJECTIVE Fangjiewan is a newly developed Chinese medicine. This is to study its Huoxuehuayu effects. METHODS The effects on hemorheology were observed in acute blood-stasis model rats，which were induced by swimming in ice water or being injected adrenalin. The effects on microcirculation were observed in mesenteric microcirculation disorder model rats induced by being injected adrenalin. And the blood clotting time were determined to evaluate the effects on PT and KPTT in rabbits. RESULTS Fangjiewan can significantly lower the animals' whole blood viscosity，plasma viscosity, blood fibrinogen concentration, ESR and platelet adhesion rate; can improve the hypercoagulable and high viscous state of blood so that avoid the appear of thrombosis; can make improvement in arterial narrowing, slow blood flow, reduction of open capillary and change of blood flow pattern; can extend the PT and KPTT time. CONCLUSION Fangjiewan has the effects on improving the hemorheology and microcirculation disorder, inhibiting either endogenous or extrinsic coagulation. The test results indicate that Fangjiewan has the Huoxuehuayu effects.